All Photos(3)
Synonym(s):
(2,4-dinitrophenyl)-hydrazine, DNPH
Linear Formula:
(O2N)2C6H3NHNH2
CAS Number:
Molecular Weight:
198.14
Beilstein:
615586
MDL number:
UNSPSC Code:
12352100
PubChem Substance ID:
NACRES:
NA.21
Recommended Products
grade
reagent grade
Quality Level
Assay
97%
contains
33% water (minimum)
mp
197-200 °C (lit.)
SMILES string
NNc1ccc(cc1[N+]([O-])=O)[N+]([O-])=O
InChI
1S/C6H6N4O4/c7-8-5-2-1-4(9(11)12)3-6(5)10(13)14/h1-3,8H,7H2
InChI key
HORQAOAYAYGIBM-UHFFFAOYSA-N
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General description
2,4-Dinitrophenylhydrazine (DNPH), a hydrazine derivative, is a potential mutagenic agent. Its molecular and crystal structure has been analyzed. The intra- and intermolecular hydrogen bonds in DNPH have been investigated by vibrational spectroscopic analysis. The mechanism of isothermal degradation of DNPH has been studied by differential scanning calorimetry (DSC).
Application
2,4-Dinitrophenylhydrazine has been used as a reagent to detect the presence of aldehydes and ketones in protein carbonyls. It may be used as a colorimetric reagent in the quantitative estimation of prednisone.
Packaging
500G contains total wet product weight of ~485G. Variance is due to addition of water to meet DOT requirements.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Desen. Expl. 1
Supplementary Hazards
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
危险化学品
Certificates of Analysis (COA)
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Kinetic analysis of isothermal decomposition of 2, 4-dinitrophenylhydrazine using differential scanning calorimetry.
Musuc AM, et al.
Thermochimica Acta, 448(2), 130-135 (2006)
J. Chem. Soc. Pak., 14, 73-73 (1992)
Journal of the American Oil Chemists' Society, 70, 881-881 (1993)
Clare L Hawkins et al.
Free radical biology & medicine, 46(8), 965-988 (2009-05-15)
Proteins are major targets for oxidative damage due to their abundance and rapid rates of reaction with a wide range of radicals and excited state species, such as singlet oxygen. Exposure of proteins to these oxidants results in loss of
Structure of 2, 4-dinitrophenylhydrazine.
Okabe N, et al.
Acta Crystallographica Section C, Crystal Structure Communications, 49(9), 1678-1680 (1993)
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