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Merck
CN

D199303

2,4-Dinitrophenylhydrazine

reagent grade, 97%, suitable for analytical testing

Synonym(s):

(2,4-dinitrophenyl)-hydrazine, DNPH

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About This Item

Linear Formula:
(O2N)2C6H3NHNH2
CAS Number:
119-26-6
Molecular Weight:
198.14
Beilstein:
615586
MDL number:
MFCD00007578
UNSPSC Code:
12352100
PubChem Substance ID:
24893586
NACRES:
NA.21
Grade:
reagent grade
Assay:
97%
Technique(s):
HPLC: suitable (for analytical testing)

Key Documents

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Product Name

2,4-Dinitrophenylhydrazine, reagent grade, 97%

grade

reagent grade

Quality Level

200

Assay

97%

contains

33% water (minimum)

technique(s)

HPLC: suitable (for analytical testing)

mp

197-200 °C (lit.)

functional group

hydrazine
nitro

SMILES string

NNc1ccc(cc1[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C6H6N4O4/c7-8-5-2-1-4(9(11)12)3-6(5)10(13)14/h1-3,8H,7H2

InChI key

HORQAOAYAYGIBM-UHFFFAOYSA-N

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General description

2,4-Dinitrophenylhydrazine (DNPH), a hydrazine derivative, is a potential mutagenic agent. Its molecular and crystal structure has been analyzed. The intra- and intermolecular hydrogen bonds in DNPH have been investigated by vibrational spectroscopic analysis. The mechanism of isothermal degradation of DNPH has been studied by differential scanning calorimetry (DSC).

Application

2,4-Dinitrophenylhydrazine has been used as a reagent to detect the presence of aldehydes and ketones in protein carbonyls. It may be used as a colorimetric reagent in the quantitative estimation of prednisone.

Packaging

500G contains total wet product weight of ~485G. Variance is due to addition of water to meet DOT requirements.

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H206,H302

Hazard Classifications

Acute Tox. 4 Oral - Desen. Expl. 1

Supplementary Hazards

EUH044

Storage Class Code

4.1A - Other explosive hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品
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Certificates of Analysis (COA)

Lot/Batch Number
0000479409
0000479409
0000481384
0000481384
0000441231

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Clare L Hawkins et al.
Free radical biology & medicine, 46(8), 965-988 (2009-05-15)
Proteins are major targets for oxidative damage due to their abundance and rapid rates of reaction with a wide range of radicals and excited state species, such as singlet oxygen. Exposure of proteins to these oxidants results in loss of
J. Chem. Soc. Pak., 14, 73-73 (1992)
Journal of the American Oil Chemists' Society, 70, 881-881 (1993)
Structure of 2, 4-dinitrophenylhydrazine.
Okabe N, et al.
Acta Crystallographica Section C, Crystal Structure Communications, 49(9), 1678-1680 (1993)
Kinetic analysis of isothermal decomposition of 2, 4-dinitrophenylhydrazine using differential scanning calorimetry.
Musuc AM, et al.
Thermochimica Acta, 448(2), 130-135 (2006)
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