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Key Documents

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Safety Information

D199303

Sigma-Aldrich

2,4-Dinitrophenylhydrazine

reagent grade, 97%

Synonym(s):

(2,4-dinitrophenyl)-hydrazine, DNPH

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About This Item

Linear Formula:
(O2N)2C6H3NHNH2
CAS Number:
119-26-6
Molecular Weight:
198.14
Beilstein:
615586
MDL number:
MFCD00007578
UNSPSC Code:
12352100
PubChem Substance ID:
24893586
NACRES:
NA.21
grade:
reagent grade

CN¥753.18

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grade

reagent grade

Assay

97%

contains

33% water (minimum)

dilution

(for analytical testing)

mp

197-200 °C (lit.)

functional group

hydrazine
nitro

SMILES string

NNc1ccc(cc1[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C6H6N4O4/c7-8-5-2-1-4(9(11)12)3-6(5)10(13)14/h1-3,8H,7H2

InChI key

HORQAOAYAYGIBM-UHFFFAOYSA-N

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This Item
PX052010594516019
Phenolphthalein indicator ACS,Reag. Ph Eur

107233

Phenolphthalein

Phenolphthalein Meets ACS Specifications GR ACS

PX0520

Phenolphthalein

Phenolphthalein ACS reagent

105945

Phenolphthalein

Phenolphthalein puriss., meets analytical specification of Ph Eur., BP, 98-101% (calc. to the dried substance)

16019

Phenolphthalein

color

white to yellow-white

color

-

color

-

color

-

form

solid

form

-

form

powder

form

solid

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

-

λmax

551-554 nm (buffer pH 9.8)

λmax

-

λmax

374 nm (2nd), 552 nm

λmax

-

storage temp.

2-30°C

storage temp.

room temp

storage temp.

room temp

storage temp.

-

General description

2,4-Dinitrophenylhydrazine (DNPH), a hydrazine derivative, is a potential mutagenic agent. Its molecular and crystal structure has been analyzed.[1] The intra- and intermolecular hydrogen bonds in DNPH have been investigated by vibrational spectroscopic analysis.[2] The mechanism of isothermal degradation of DNPH has been studied by differential scanning calorimetry (DSC).[3]

Application

2,4-Dinitrophenylhydrazine has been used as a reagent to detect the presence of aldehydes and ketones in protein carbonyls.[4][5][6] It may be used as a colorimetric reagent in the quantitative estimation of prednisone.[7]

Packaging

500G contains total wet product weight of ~485G. Variance is due to addition of water to meet DOT requirements.

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H206,H302

Hazard Classifications

Acute Tox. 4 Oral - Desen. Expl. 1

Supplementary Hazards

EUH044

Storage Class Code

4.1A - Other explosive hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品

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Certificates of Analysis (COA)

Lot/Batch Number
0000412358
0000412358
0000409137
0000409138
0000400119

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Vibrational spectroscopy and theoretical studies on 2, 4-dinitrophenylhydrazine.
Chis V, et al.
Journal of Molecular Structure, 744, 363-368 (2005)
J. Chem. Soc. Pak., 14, 73-73 (1992)
Clare L Hawkins et al.
Free radical biology & medicine, 46(8), 965-988 (2009-05-15)
Proteins are major targets for oxidative damage due to their abundance and rapid rates of reaction with a wide range of radicals and excited state species, such as singlet oxygen. Exposure of proteins to these oxidants results in loss of
Journal of the American Oil Chemists' Society, 70, 881-881 (1993)
Chem. Abstr., 118, 22127d-22127d (1993)
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