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809705

Sigma-Aldrich

5-DBCO-PEG4-dUTP, 10mM Aqueous Solution

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Synonym(s):
5-DBCO-PEG4-dUTP
Empirical Formula (Hill Notation):
C42H54N5O21P3
Molecular Weight:
1057.82
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.22

Assay

>95% (HPLC)

Quality Level

form

solution

reaction suitability

reaction type: click chemistry

solubility

Tris buffer: 100 mM (pH 7.5)

shipped in

wet ice

storage temp.

−20°C

SMILES string

O=C1NC(N([C@H]2C[C@@H]([C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O2)O)C=C1/C=C\CNC(CCOCCOCCOCCOCCNC(CCC(N3CC4=CC=CC=C4C#CC5=CC=CC=C53)=O)=O)=O)=O

Application

5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR with Taq polymerase. It is enzymatically incorporated into DNA/cDNA as a substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can be labeled with azide-labeled molecules using copper-free click chemistry. This enables experiments such as introduction of a biotin group for purification tasks, introduction of a fluorescent group for detection, or crosslinking the DNA to azide-functionalized biomolecules such as proteins.

WGK

WGK 2


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