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T0377

Sigma-Aldrich

Tricine

≥99% (titration)

Synonym(s):

N-[Tris(hydroxymethyl)methyl]glycine

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About This Item

Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS Number:
5704-04-1
Molecular Weight:
179.17
Beilstein:
1937804
EC Number:
227-193-6
MDL number:
MFCD00004277
UNSPSC Code:
12161700
PubChem Substance ID:
24899884
NACRES:
NA.25

Quality Level

400

Assay

≥99% (titration)

form

crystalline powder

useful pH range

7.4-8.8

pKa (25 °C)

8.1

mp

187 °C

solubility

water: 0.25 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

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Expand™ High FidelityPLUS PCR System, dNTPack sufficient for ≤125 reactions (04743725001), sufficient for ≤500 reactions (04743733001), suitable for PCR, dNTPs included

EHIFIPNRO

Expand™ High FidelityPLUS PCR System, dNTPack

Expand™ 高保真PLUS PCR 系统 sufficient for ≤500 reactions (03300226001), sufficient for ≤2,500 reactions (03300234001), suitable for PCR, High Fidelity PCR, hotstart: no, dNTPs included: no

EHIFIPRO

Expand™ 高保真PLUS PCR 系统

Expand™ 高保真PCR系统，dNTPack suitable for PCR, High Fidelity PCR, hotstart: no, dNTPs included

EHIFIDNTPRO

Expand™ 高保真PCR系统，dNTPack

Expand™ 高保真PCR系统 sufficient for ≤40 reactions (11732641001), sufficient for ≤200 reactions (11732650001), sufficient for ≤1,000 reactions (11759078001)

EHIFIRO

Expand™ 高保真PCR系统

grade

Molecular Biology

grade

-

grade

-

grade

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

form

liquid

form

-

form

-

form

-

packaging

pkg of 125 U (04743725001), pkg of 500 U (04743733001 [2x250 U])

packaging

pkg of 2,500 U (03300234001 [10x250 U]), pkg of 500 U (03300226001 [2x250 U])

packaging

pkg of 2,500 U (04738276001 [10x250 U]), pkg of 100 U (04738250001), pkg of 500 U (04738268001 [2x250 U])

packaging

pkg of 2,500 U (11759078001 [10x250 U]), pkg of 100 U (11732641001), pkg of 500 U (11732650001 [2x250 U])

solubility

water: miscible

solubility

-

solubility

-

solubility

-

General description

Tricine is the most frequently used electrophoresis buffer. It is also useful for the resuspension of cell pellets. Tricine functions as a trailing ion and aids the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems. It has a lower negative charge than glycine, which helps it migrate faster. Tricine has a high ionic strength that allows increased ion movement and less protein movement. This in turn leads to the separation of low molecular weight proteins in lower percent acrylamide gels.[1]

Application

Buffer component for separation of low molecular weight peptides.
Tricine has been used:
  • to prevent precipitation of salts during autoclaving of Emiliania huxleyi cultures[2]
  • as a component of buffer A for the homogenization of samples like Caenorhabditis elegans, Drosophila, and plants[3]
  • as a component of fresh assay buffer to measure serum melatonin by radioimmunoassay[4]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000446748
    0000446747
    0000446748
    0000446747
    0000436758

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Assessing the applicability of Emiliania huxleyi coccolith morphology as a sea-surface salinity proxy
    Fielding SR, et al.
    Limnology and Oceanography, 54(5), 1475-1480 (2009)
    Encyclopedia of Dairy Sciences (2011)
    Elzbieta Turkowska et al.
    Chronobiology international, 31(1), 134-143 (2013-10-19)
    The avian pineal gland, apart from the hypothalamic master clock (suprachiasmatic nuclei, SCN) and retina, functions as an independent circadian oscillator, receiving external photic cues that it translates into the rhythmical synthesis of melatonin, a biochemical signal of darkness. Functional
    Yunfeng Ding et al.
    Nature protocols, 8(1), 43-51 (2012-12-12)
    The lipid droplet (LD) is a cell organelle that has been linked to human metabolic syndromes and that can be exploited for the development of biofuels. The isolation of LDs is crucial for carrying out morphological and biochemical studies of
    Arpita Gantayet et al.
    Biofouling, 29(1), 77-85 (2012-12-06)
    The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
    View All Related Papers

    Protocols

    Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

    To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

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