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Key Documents

Safety Information

SH0421

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, DNA

Phosphatases

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25 MG
¥607.22
100 MG
¥1,942.18
250 MG
¥3,312.50
500 MG
¥6,784.86
1 G
¥9,530.86
5 G
¥33,205.25

¥607.22

List Price¥714.37Save 15%

Available to ship on2025年4月25日Details

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25 MG
¥607.22
100 MG
¥1,942.18
250 MG
¥3,312.50
500 MG
¥6,784.86
1 G
¥9,530.86
5 G
¥33,205.25

About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

¥607.22

List Price¥714.37Save 15%

Available to ship on2025年4月25日Details

New, lower price on this item!

Request a Bulk OrderRequest more information

Quality Level

product line

MISSION®

storage temp.

−20°C

Gene Information

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SH1821SH1021SH0821
Gene Information

Gene Information

Gene Information

Gene Information

product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) are provided as purified plasmid DNA suitable for virus production and transient transfection. DNA is provided in 40 μl aliquots per well in Tris,EDTA (TE) solution. An average of 2 μg per clone is provided per well with a range from 400 ng to 4 μg. The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Other Notes

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 317, Number of Clones: 2065
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Regulatory Information

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Michel L Tremblay et al.
Cell metabolism, 7(2), 101-103 (2008-02-06)
FoxO transcription factors contribute to cardiac muscle remodeling and insulin signaling, but how they link insulin resistance and maladaptive heart hypertrophy remains unknown. A new study by Ni et al. (2007) shows that sustained activation of FoxO1 or FoxO3 in
Wiljan J A J Hendriks et al.
The FEBS journal, 275(5), 816-830 (2008-02-27)
Some 40-odd genes in mammals encode phosphotyrosine-specific, 'classical' protein tyrosine phosphatases. The generation of animal model systems and the study of various human disease states have begun to elucidate the important and diverse roles of protein tyrosine phosphatases in cellular

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