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Safety Information

I3911

Sigma-Aldrich

Protease Inhibitor Cocktail

Animal-Free, lyophilized powder, for the inhibition of serine, cysteine, aspartic and metalloproteases, for general use, lyophilized powder

Synonym(s):

protease inhibitor

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5 X 10 ML
CN¥13,241.17

CN¥13,241.17

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5 X 10 ML
CN¥13,241.17

About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
form:
lyophilized powder
solubility:
water: soluble
storage temp.:
−20°C

CN¥13,241.17

Please contact Customer Service for Availability

Product Name

Protease Inhibitor Cocktail, Animal Component Free, for general use, lyophilized powder

Quality Level

200

form

lyophilized powder

solubility

water: soluble

storage temp.

−20°C

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General description

Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).
This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid-proteases, and aminopeptidases.

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Application

Animal component free (ACF). Optimized and tested for general use. Protease Inhibitor Cocktail, Animal Component Free has been used to prevent protein lysis in saliva samples,[1] peritoneal residential macrophage cells[2] and in embryo culture medium.[3]

Components

AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin

Quantity

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Physical form

Lyophilized powder that is water soluble.
The components of this ACF cocktail do not originate from animal sources and can thus be used in the production of animal free proteins.

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1A

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

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    Certificates of Analysis (COA)

    Lot/Batch Number
    099M4056V
    11190304
    076M4124V
    11160605
    072M4051V

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    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

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    Early protein profile of human embryonic secretome.
    Foresta C, et al.
    Frontiers in Bioscience, 21, 620-634 (2016)
    Carlo Foresta et al.
    Frontiers in bioscience (Landmark edition), 21, 620-634 (2015-12-29)
    Embryos obtained by in vitro fertilization are currently assessed by morphology, but displays limitations with over 70% of embryos failing to implant. In this study, we performed HPLC-MS/MS analysis on the conditioned medium obtained from 50 human embryos at the
    Justin de Boer et al.
    Journal of pediatric gastroenterology and nutrition, 71(4), e109-e112 (2020-09-23)
    The gold standard diagnostic procedure for food protein-induced proctocolitis (FPIP) requires flexible sigmoidoscopy (FS). To date there is no validated, noninvasive test to confirm FPIP diagnosis. Eosinophil-derived neurotoxin (EDN), a product of eosinophil (EOS) degranulation, has been shown to correlate
    Preliminary analysis of the protein profile in saliva during physiological term and preterm delivery
    Lopucki M, et al.
    Molecular Medicine Reports, 17(6), 8253-8259 (2018)
    Marija Holcar et al.
    Scientific reports, 10(1), 21346-21346 (2020-12-09)
    Human plasma is a complex fluid, increasingly used for extracellular vesicle (EV) biomarker studies. Our aim was to find a simple EV-enrichment method for reliable quantification of EVs in plasma to be used as biomarker of disease. Plasma of ten
    View All Related Papers

    Related Content

    Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

    Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service