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E8158

Sigma-Aldrich

pFLAG-ATS™ Expression Vector

Bacterial vector for periplasmic expression of N-terminal FLAG fusion proteins

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About This Item

UNSPSC Code:
12352200
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tag

FLAG® tagged

grade

Molecular Biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

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Anti-Tumor Necrosis Factor-α antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

T8300

Anti-Tumor Necrosis Factor-α antibody produced in rabbit

抗-肿瘤坏死因子-α 山羊抗 affinity isolated antibody, lyophilized powder

T1816

抗-肿瘤坏死因子-α 山羊抗

单克隆抗 肿瘤坏死因子受体 II 小鼠抗 clone 22221, purified immunoglobulin, lyophilized powder

T1815

单克隆抗 肿瘤坏死因子受体 II 小鼠抗

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

clone

TN3-19.12, monoclonal

clone

polyclonal

clone

polyclonal

clone

22221, monoclonal

species reactivity

rat, mouse

species reactivity

human

species reactivity

pig

species reactivity

human

Gene Information

mouse ... Tnf(21926)
rat ... Tnf(24835)

Gene Information

human ... TNF(7124)

Gene Information

pig ... TNF(397086)

Gene Information

human ... TNF(7124)

General description

The pFLAG-ATS™ Expression Vector is a 5.4 kb E. coli expression vector used for cloning and periplasmic expression of properly inserted open reading frame as an N-terminal FLAG fusion protein. The N-terminal FLAG® fusion protein may be detected using Monoclonal ANTI-FLAG M1, Catalog Number F3040, or Monoclonal ANTI-FLAG M2, Catalog Number F3165, or purified using ANTI-FLAG M1 Agarose Affinity Gel, Catalog Number A4596 or ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.

The pFLAG-ATS-BAP Control Plasmid is a 6.8 kb E. coli plasmid used for efficient and controlled periplasmic expression of N-terminal FLAG-BAP fusion protein.

Vector Maps and Sequences

Components

  • pFLAG-ATS™ Expression Vector 10 μg ( E5769) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA
  • pFLAG-ATS™-BAP Control Plasmid 1 μg (C9079) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-ATS is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

Regulatory Information

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S Davletova et al.
Journal of experimental botany, 52(355), 215-221 (2001-04-03)
A calmodulin like domain protein kinase (CPK) homologue was identified in alfalfa and termed MsCPK3. The full-length sequence of cDNA encoded a 535 amino acid polypeptide with a molecular weight of 60.2 kDa. The deduced amino acid sequence showed all
J L Gommerman et al.
Blood, 91(6), 1891-1900 (1998-04-16)
Steel factor (SLF), the ligand for the c-Kit receptor, protects hemopoietic progenitors and mast cells from apoptosis. We show here that protection of 32D-Kit cells or mast cells from apoptosis by SLF is abrogated through concurrent inhibition of Ca2+ influx.
Miaoying Tian et al.
BMC biochemistry, 6, 15-15 (2005-08-25)
Kazal-like serine protease inhibitors are defined by a conserved sequence motif. A typical Kazal domain contains six cysteine residues leading to three disulfide bonds with a 1-5/2-4/3-6 pattern. Most Kazal domains described so far belong to this class. However, a
Birendra Singh et al.
Microbiology (Reading, England), 154(Pt 3), 797-809 (2008-03-04)
We describe an ATP-binding cassette (ABC) transporter in Pseudomonas putida KT2440 that mediates the uptake of glutamate and aspartate. The system (AatJMQP, for acidic amino acid transport) is encoded by an operon involving genes PP1071-PP1068. A deletion mutant with inactivated
P J Brown et al.
FEBS letters, 497(1), 31-38 (2001-05-30)
Micronemes are specialised organelles, found in all apicomplexan parasites, which secrete molecules that are essential for parasite attachment to and invasion of host cells. Regions of several microneme proteins have sequence similarity to the Apple domains (A-domains) of blood coagulation
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