pSF-T7LacO-NH2-FLAG

The pSF-T7LacO-NH2-FLAG expression vector is a 5.5 kb E. coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as an N-terminal Met-FLAG fusion protein. The FLAG epitope is a small- hydrophilic- 8 amino acid-tag (DYKDDDDK) that enables sensitive detection and high quality protein purification using anti-FLAG products. The N-terminal Met-FLAG fusion protein may be detected using monoclonal anti-FLAG M1 (catalogue number F3040) or M2 (F3165) antibodies. The fusion protein may be purified using anti-FLAG affinity gel (A2220). In this vector, transcription of the fusion protein is driven by the strong phage T7 promoter. This requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells (catalogue number B2935).

Cloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.Multiple cloning site notes:In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.

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Oxford Genetics is a trademark of Oxford Genetics Ltd