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Key Documents

Safety Information

E7033

Sigma-Aldrich

pFLAG-CMV-2 Expression Vector

shuttle vector for intracellular transient expression of N-terminal Met-FLAG

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200 μG
CN¥6,882.71

CN¥6,882.71


Estimated to ship onMay 12, 2025Details

A recombinant, preservative-free antibody is available for your target. Try ZMS1039


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200 μG
CN¥6,882.71

About This Item

UNSPSC Code:
12352200

CN¥6,882.71


Estimated to ship onMay 12, 2025Details

A recombinant, preservative-free antibody is available for your target. Try ZMS1039

tag

FLAG® tagged

grade

Molecular Biology

form

buffered aqueous solution

quality

shuttle vector for intracellular transient expression of N-terminal Met-FLAG

Peptide tag location

N-terminal

shipped in

dry ice

storage temp.

−20°C

General description

The pFLAG-CMV-2 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG® fusion proteins in mammalian cells.

pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.

The pFLAG-CMV-2-BAP Control Plasmid is a 6.1 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.

Vector Maps and Sequences

Components

  • pFLAG-CMV-2 Expression Vector 20 μg (E7398) is supplied as 0.5 mg/ml in 10 mM Tris-HCl pH 8.0 in 1 mM EDTA.
  • pFLAG-CMV-2-BAP Control Plasmid 20 μg (P5100) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Principle

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

Regulatory Information

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