E7033
pFLAG-CMV-2 Expression Vector
shuttle vector for intracellular transient expression of N-terminal Met-FLAG
tag
FLAG® tagged
grade
Molecular Biology
form
buffered aqueous solution
quality
shuttle vector for intracellular transient expression of N-terminal Met-FLAG
peptide tag location
N-terminal
shipped in
dry ice
storage temp.
−20°C
General description
The pFLAG-CMV™-2 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG® fusion proteins in mammalian cells.
pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.
The pFLAG-CMV-2-BAP Control Plasmid is a 6.1 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
Vector Maps and Sequences
pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.
The pFLAG-CMV-2-BAP Control Plasmid is a 6.1 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
Vector Maps and Sequences
Biochem/physiol Actions
The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs.
Other Notes
- pFLAG-CMV™-2 Expression Vector 20 μg (E7398) is supplied as 0.5 mg/ml in 10 mM Tris-HCl pH 8.0 in 1 mM EDTA.
- pFLAG-CMV™-2-BAP Control Plasmid 20 μg (P5100) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Legal Information
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
Regulatory Information
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Instructions
pFLAG-CMV-1 and pFLAG-CMV-2 Vector Maps
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