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D6063

Sigma-Aldrich

REDTaq® SuperPak™ DNA Polymerase

Taq for routine PCR with inert dye; with 10X buffer & dNTP mix

Synonym(s):

DNA polymerase with dNTP, DNA polymerase with loading dye, Taq DNA polymerase

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1 PKG
CN¥5,586.77

CN¥5,586.77

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1 PKG
CN¥5,586.77

About This Item

UNSPSC Code:
41106300
NACRES:
NA.55

CN¥5,586.77

Please contact Customer Service for Availability

Quality Level

200

form

liquid

usage

sufficient for 250 reactions

feature

dNTPs included
hotstart: no

concentration

1 unit/μL

technique(s)

PCR: suitable

color

red

input

purified DNA

suitability

suitable for PCR

shipped in

wet ice

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Kromasil® C18 HPLC Column 5 μm particle size, pore size 100 Å, L × I.D. 250 mm × 4 mm

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Kromasil® C18 HPLC Column 5 μm particle size, pore size 100 Å, L × I.D. 100 mm × 21.2 mm

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particle size

5 μm

particle size

3.5 μm

particle size

5 μm

particle size

5 μm

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

product line

Kromasil®

product line

Kromasil®

product line

Kromasil®

product line

Kromasil®

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

General description

REDTaq® SuperPak™ DNA Polymerase is Sigma′s Taq DNA Polymerase mixed with an inert red dye. REDTaq® SuperPak™ DNA Polymerase is a convenient package, that includes all the necessary components for a PCR reaction except primers, DNA template and water. It consists of Sigma′s high quality REDTaq DNA polymerase, 10 mM ultrapure deoxynucleotide mix and 10´ PCR reaction buffer. The dye provides quick recognition of reactions to which enzyme has been added as well as visual confirmation of complete mixing.

Application

REDTaq® SuperPak™ DNA Polymerase has been used in:
  • reverse transcriptase-polymerase chain reaction (RT-PCR)[1]
  • nested PCR[2]
  • routine PCR amplification[3]

Features and Benefits

  • Contains inert dye that enables visual confirmation of enzyme addition and direct loading onto agarose gel without the need for an additional loading dye
  • Suitable for nested PCR
  • Contains ultrapure 10mM dNTP mix in a separate vial

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
SuperPak is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included
  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCJ5011
    SLCH8736
    SLCD2747
    SLBX4809
    SLBX4808

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    Find documentation for the products that you have recently purchased in the Document Library.

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    M Yeste-Velasco et al.
    Neuroscience, 159(3), 1135-1147 (2009-04-10)
    Increasing evidence implicates the c-Jun NH(2)-terminal kinase (JNK) pathway in the regulation of apoptosis in neurodegenerative diseases. In this study, we examined the neuroprotective effect of SP600125, a selective JNK inhibitor, in cerebellar granule cells (CGNs) deprived of serum and
    A Jóźwik et al.
    Veterinary research communications, 29(4), 347-359 (2005-03-09)
    Two pairs of primers were prepared, both localized within the sequences of the nucleoprotein gene (NP) of canine distemper virus (CDV). A number of experiments were done to optimize the conditions of RT-PCR and nested PCR methods. The nucleic acids
    Vishnu Chintalgattu et al.
    Journal of molecular and cellular cardiology, 35(3), 277-286 (2003-04-05)
    Vascular endothelial growth factor (VEGF), produced predominantly by endothelial cells, is involved in angiogenesis and mitogenesis. Myofibroblasts (myoFb) are phenotypically transformed fibroblast-like cells found at the site of myocardial infarction. Since myoFb play a role in tissue repair/remodeling at the
    M Yeste-Velasco et al.
    Neuropharmacology, 53(2), 295-307 (2007-07-07)
    Recent studies have demonstrated that neuronal reentry in the cell cycle and specifically the expression of the transcription factor E2F-1, constitutes a pathway that may be involved in neuronal apoptosis after serum and potassium withdrawal. Other enzymes such as glycogen
    D Alvira et al.
    Neuroscience, 147(3), 746-756 (2007-06-23)
    The mechanism involved in neuronal apoptosis is largely unknown. Studies performed on neuronal cell cultures provide information about the pathways which orchestrate the process of neuronal loss and potential drugs for the treatment of neurological disorders. In the present study
    View All Related Papers

    Protocols

    Applications with REDTaq™

    Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

    Amplification of Genomic DNA using REDTaq® DNA Polymerase

    Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service