Immune Response

Measuring Immune Response to Monoclonal Antibody (mAb) Therapies

Understanding and accurately measuring how the immune system responds to monoclonal antibody (mAb) therapeutics is essential for evaluating mechanisms of action, optimizing dosing, ensuring functional potency, and supporting long-term safety assessment. mAbs exert their effects through targeted recognition and immune mechanisms, including cell signaling modulation, receptor blockade, and effector-cell engagement. Integrating multiplex immunoassays, cell-based assays, and advanced molecular profiling allows comprehensive characterization of both the biological activity of mAbs and the immune responses they trigger, informing candidate selection, dose optimization, and prediction of clinical outcomes across diverse therapeutic areas, including oncology, immunology, autoimmune disease, and infectious disease.

Comprehensive PK/PD and Immunogenicity Analysis

Monitor preclinical responses with MILLIPLEX^® multiplex biomarker assays, powered by Luminex^® xMAP^® technology for high-precision, multi-analyte profiling. Our industry-leading MILLIPLEX^® panels enable quantitative investigation of cytokines, chemokines, growth factors, complement and immunoglobulins, measuring dozens of biomarkers in parallel with speed and sensitivity. For ultra-sensitive detection of biotherapeutic pharmacokinetics, pharmacodynamics, and immunogenicity, use SMC^® technology for single molecule-level resolution. These platforms support robust quantitative analysis critical for immune response monitoring, PK/PD modeling, and immunogenicity risk assessment.

Evaluating Cellular Immunity in mAb Development

Therapeutic antibodies frequently depend on cellular immune mechanisms for meaningful clinical responses. Key processes include T cell activation, NK cell–mediated cytotoxicity, macrophage phagocytosis, pathway inhibition, and immune cell recruitment. Accurately measuring these effects provides critical insights into potency, selectivity, and therapeutic safety.

Our suite of analytical tools and assays for cellular immunity includes:

• ELISpot Assays: Detect cytokine-secreting T cells (e.g., IFNγ, IL-2) in response to mAb treatment.
• Multiplex Immunoassays: Profile mAb-induced cytokines and inflammatory mediators such as TNFα, IL-6, IL-8, IL-1β, and IFNγ for a comprehensive immune activation snapshot.
• Western Blot: Confirm modulation of intracellular signaling pathways downstream of mAb engagement.
• qPCR: Quantify gene expression changes related to immune modulation, stress responses, or cell-state changes elicited by mAb exposure.
• Single-Cell RNA Sequencing (scRNA-seq): Dissect cell-specific transcriptional signatures and heterogeneity at single-cell resolution in response to mAb therapy.
• Duolink^® PLA (Proximity Ligation Assay): Visualize protein–protein interactions, receptor engagement, or pathway activation within cells and tissues for mechanistic and biomarker studies.

For Research Use Only. Not For Use In Diagnostic Procedures.

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