亲和色谱树脂
亲和色谱通过高度选择性生物化学相互作用来分离复杂的混合物。这种应用广泛的纯化技术可高效地分离目标分子和杂质。我们的亲和树脂系列是为了实现高通量和经济有效的纯化过程而设计,可用于从工艺开发到大规模生产的过程。
相关资源
- Tool: mAbsizer™ Calculator
mAb Expression, Purification, and Formulation
- PDF: Biochromatography Portfolio Guide
Bulk Resin, Membranes and Prepacked Columns
- PDF: Chromatography Purification Selection Guide
Chromatography product selection guide.
- Data Sheet: Eshmuno® A Resin
Eshmuno® A media is a rigid, high-capacity, acid and alkalineresistant Protein A affinity chromatography media for the purification of Fc-containing proteins, including but not limited to monoclonal antibodies.
- Data Sheet: Eshmuno® P anti-A and anti-B Resins
High performance, acid and alkaline resistant affinity chromatography resins designed for the removal of anti-A and anti-B antibodies from plasma-derived immunoglobulin (Ig).
- Data Sheet: Fractogel®Chromatography Resin
Fractogel® resins consist of synthetic methacrylate based polymeric beads providing excellent pressure stability resulting in high flow rates. Depending on your purification application, we can offer resin sized M-type beads with a particle size of 40–90 μm and small S-type beads with a particle size in the range of 20–40 μm.
- Application Note: Using Ethylene Glycol Emprove® Evolve to increase Eshmuno®
Sodium hydroxide (NaOH) is a standard cleaning agent for protein A chromatography resins. Typically, a concentration of 0.1 mol/L NaOH is used. The inclusion of 20% ethylene glycol in a 0.1 mol/L NaOH cleaning solution increases the lifetime of Eshmuno® A columns significantly.
- Application Note: Detection and Quantification of Leached Protein A from Eshmuno® A Resin Using Cygnus F400 Protein A ELISA Kit
Cygnus Technologies performed the initial feasibility testing of the Eshmuno® A Protein A ligand in the Cygnus Protein A ELISA Kit (Cat# F400). Upon successful completion of the feasibility testing, Cygnus proceeded with quantification of the assay for use in in-process and drug substance samples using the Eshmuno® A Protein A resin.
- User Guide: MiniChrom® Pre-Packed Columns
The MiniChrom® columns are prepacked chromatography columns designed for laboratory-scale process development evaluations. When used for biomolecule separations, the results achieved with MiniChrom® columns are typically similar to those obtained using traditional laboratory-scale columns from different vendors.
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