Affinity Chromatography Resins

Affinity chromatography is a highly selective purification technique that separates biomolecules through specific biological interactions. It is commonly used to capture target molecules while reducing impurities early in downstream processing.

Our affinity chromatography portfolio supports multiple modalities  including capture of monoclonal antibodies (mAb) and antibody derivatives, adeno-associated virus (AAV) purification, plasma purification and affinity purification of recombinant proteins.

Selection depends on your target molecule, process format, productivity goals, and downstream requirements. Key considerations include molecule type, binding mechanism, process intensification strategy, throughput targets, and scalability requirements.

For mAb capture, our Protein A portfolio offers multiple options depending on process goals:

• Amsphere^® A+ is the resin of choice for high-titer or intensified processes that demand maximum column productivity. Engineered for outstanding dynamic binding capacity at low residence times, it enables high-throughput capture without sacrificing binding efficiency. Its exceptional caustic stability and long cycle lifetime ensure reliable performance across extended resin reuse, meeting the process demands of both intensified batch and continuous manufacturing modes.
• Amsphere^®A3 Protein A is an established platform resin with consistent, reproducible Protein A capture performance across a broad range of mAbs and their derivatives. This resin is a proven choice for dual sourcing strategies, offering a qualifiable alternative that reduces single-supplier risk. For processes that require a dependable, scalable foundation for Protein A capture, Amsphere A3 is a reliable choice.
• Eshmuno^® A delivers reliable Protein A capture performance even under the most demanding feed conditions, including high-aggregate feedstocks, multispecific molecules, and other complex biological formats. The resin’s strong pressure-flow properties enable operation at high linear flow rates, while the selectivity profile supports effective impurity removal, minimizing the requirements for downstream polishing steps.

For intensified mAb capture processes, Amsphere A+ is the recommended choice. It is specifically designed for high-titer and intensified processing modes, including concentrated fed-batch and continuous bioprocessing, delivering outstanding dynamic binding capacity at low residence times. Its exceptional caustic stability and long cycle lifetime make it well-suited for the extended resin reuse demands of both intensified batch and continuous manufacturing operations.

Amsphere^® A+ and Amsphere^® A3 are both designed for standard to high-titer mAb capture processes, but serve different manufacturing strategies. Amsphere^® A+ is optimized for maximum productivity in intensified and high-titer processes, offering outstanding dynamic binding capacity at low residence times combined with exceptional caustic stability and long cycle lifetime, making it ideal for concentrated fed-batch and continuous bioprocessing platforms. Amsphere^® A3 is an established platform resin offering consistent, reproducible performance across a broad range of mAbs and derivatives, with a well-balanced productivity and cost-of-goods profile, and is a proven choice for dual sourcing strategies.

Eshmuno^® A addresses a different set of process challenges. It is the resin of choice when feed complexity, high aggregate levels, multispecific molecules, or high flow rate requirements define the process. Its strong pressure-flow properties allow operation at high linear flow rates, and its selectivity profile supports effective impurity removal, minimizing the requirements for downstream polishing steps.

Eshmuno^® AAV9 affinity resin is designed specifically for affinity capture of AAV9 viral vectors including wild-type AAV9 and AAV9-derived engineered capsids. Other serotype-specific resins can be accommodated via our custom-affinity Eshmuno^® Fit platform.

Eshmuno^® FIT is designed for difficult-to-purify molecules that require a custom affinity capture strategy. It is especially useful when a standard Protein A template cannot be applied, such as for targets without an Fc region. By using a custom ligand to selectively capture stable molecules, Eshmuno^® FIT can help simplify downstream purification, reduce the number of purification steps, improve yields, and lower cost of goods. It can be used for applications such as non-traditional mAbs, peptides, enzymes, vaccines, and AAV vectors, and may also help establish a purification template for similar molecules.