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安全信息

E9644

Sigma-Aldrich

重组人表皮细胞生长因子

EGF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

别名:

表皮生长因子 人, EGF

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About This Item

CAS号:
62253-63-8
MDL编号:
MFCD00132565
UNSPSC代码:
12352202
NACRES:
NA.77

生物来源

human

质量水平

300

重组

expressed in E. coli

检测方案

≥97% (SDS-PAGE)

形式

lyophilized powder

效能

0.08-0.8 ng/mL ED50/EC50

分子量

~6 kDa

包装

pkg of 5X0.2 mg
pkg of 0.2 mg
pkg of 0.5 mg

储存条件

avoid repeated freeze/thaw cycles

技术

cell culture | mammalian: suitable

杂质

≤1 EU/μg endotoxin (EGF)

颜色

white

溶解性

water: soluble, clear, colorless

UniProt登记号

P01133

储存温度

−20°C

基因信息

human ... EGF(1950)

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应用

该产品用作促分裂原，在各种细胞系中引发细胞开始有丝分裂。在组织培养中，EGF可用于减少或消除对血清的需求，并可与其他培养基添加剂和激素一起使用。

生化/生理作用

表皮生长因子（EGF）是在许多哺乳动物物种中的大量组织和体液中都存在的一种小型促有丝分裂多肽。EGF是生长因子家族的一员，其特征在于形成了三个二硫键的6个保守半胱氨酸基序。EGF对多种表皮和上皮细胞具有促有丝分裂作用，包括成纤维细胞、神经胶质细胞、血管和角膜内皮细胞、牛颗粒细胞、HeLa细胞、SV40-3T3细胞和乳腺上皮细胞。
受EGF影响的细胞功能：有丝分裂、离子通量、葡萄糖转运、糖酵解、核酸及蛋白质合成、存活、生长、增殖和分化。
EGF的生物学影响：抑制胃酸分泌、胎儿生长发育和中枢神经系统的神经调节。
受EGF影响的通路：EGFR信号通路，MAPK级联反应通路，PIP，Ca+2信号通路

组分

人EGF（hEGF）是与β-尿抑胃素相同的分子，而这是一种基于其能够抑制胃酸分泌的能力而分离的分子。EGF在结构上与人转化生长因子-α同源，而二者都可以通过EGF受体发挥其作用。 E9644是天然成熟EGF的N-末端甲硫氨酸形式。

注意

该产品应当储存在-20°C并将保持活性长达两年。在重悬后，它可在2至-8°C下储存一个月，或在分装冷冻后在-70至-20℃下储存更长时间。

制备说明

该产品是由经0.2 μm-过滤的pH 7.4磷酸盐缓冲液冻干而得。它应该通过使用10mM 乙酸将小瓶中的内容物加入至1mg / mL来进行重悬。 如需稀释或更低的浓度，但不低于10 μg/mL时，需要加入0.1% BSA或HSA。如用于增殖检测，则可通过不含白蛋白的培养基对其进一步稀释。

分析说明

该产品的生物学活性是通过在BALB/MK细胞中进行的增殖试验而得以测量的。EC50被定义为在基于细胞的生物测定中可引起细胞生长增加50％的生长因子有效浓度。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. What is the difference between Product No. E9644 and E4269, Epidermal Growth Factor?

    Product No. E9644 is the mature EGF protein. Long EGF (Product No. E4269) is an analog of epidermal growth factor comprising the human EGF amino acid sequence plus a 53 amino acid N-terminal extension peptide. Long EGF has been developed as an inexpensive, high quality potent analog of EGF for use as a growth factor supplement for serum-free or low-serum cell culture.

  3. What is the molecular weight of Epidermal Growth Factor (EGF) human, Product E9644?

    The mature protein has a molecular weight of 6 kD.

  4. How long can I store a solution of Epidermal Growth Factor (EGF) human, Product E9644?

    Solution stability can be found on the product data sheet. If Product No. E9644 has been reconstituted with 0.2 micron-filtered 10 mM acetic acid containing 0.1% HSA or BSA to a concentration of not less than 10 ug/ml, it can be stored at 2-8 °C for a maximum of one month. For extended storage, freeze in working aliquots at -70 °C or -20 °C. Repeated freezing and thawing is not recommended.

  5. Can I use PBS to reconstitute Epidermal Growth Factor (EGF) human, Product E9644?

    We recommend reconstitution with acetic acid to make sure the entire product will be solubilized.  PBS can be used, but the total amount of the protein in the vial may not go into solution.  It is best to make a higher concentration in acetic acid, and then dilute into PBS.

  6. Can Epidermal Growth Factor (EGF) human, Product E9644, be used on rat cells?

    This product has been tested for activity using the mouse Balb/3T3 cell line.  We have not tested its activity in rat cell culture, but we expect that will also work in that application.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Piera Versura et al.
Investigative ophthalmology & visual science, 52(8), 5488-5496 (2011-04-19)
To investigate the immune response of human conjunctival epithelium to hyperosmolar stress. Tear osmolarity was measured in 15 normal subjects and 25 dry eye (DE) patients; conjunctival imprint cytology samples were obtained at the nasal bulbar area. Subconfluent primary human
Anita Alexa et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(9), 2711-2716 (2015-03-03)
Mitogen-activated protein kinases (MAPKs) bind and activate their downstream kinase substrates, MAPK-activated protein kinases (MAPKAPKs). Notably, extracellular signal regulated kinase 2 (ERK2) phosphorylates ribosomal S6 kinase 1 (RSK1), which promotes cellular growth. Here, we determined the crystal structure of an
Koji Teramoto et al.
Cellular signalling, 62, 109329-109329 (2019-06-04)
EphA2, which belongs to the Eph family of receptor tyrosine kinases, is overexpressed in a variety of human cancers. Serine 897 (S897) phosphorylation of EphA2 is known to promote cancer cell migration and proliferation in a ligand-independent manner. In this
Hossein Baharvand et al.
Molecular vision, 13, 1711-1721 (2007-10-26)
A new strategy of treating ocular surface reconstruction is to transplant a bioengineered graft by expanding limbal stem cells (SCs) ex vivo on the amniotic membrane (AM). The reasons for the exceptional success on the AM are not fully understood
Marzeih Ebrahimi et al.
Molecular vision, 16, 1680-1688 (2010-09-02)
The aim of this study is to create an ex vivo model to examine the expression of major heat-shock protein (HSP) families; HSP60, HSP72, and HSP90, and heat-shock cognate 70 (HCS70) at the mRNA and protein level in differentiating corneal
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