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Merck
CN

767964

Sigma-Aldrich

细胞游离氨基酸混合物 - 13C,15N

5-100 mM in water, 98 atom % 15N, 98 atom % 13C

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About This Item

UNSPSC代码:
12352209
NACRES:
NA.12

同位素纯度

98 atom % 13C
98 atom % 15N

质量水平

形式

solid

浓度

5-100 mM in water

技术

bio NMR: suitable
protein expression: suitable

运输

dry ice

储存温度

−20°C

应用

该产品适合作为无细胞蛋白质合成的稳定同位素标记氨基酸的来源。对于质谱应用,考虑产品909653,用于质谱的稳定同位素标记氨基酸混合物。

由于酪氨酸的浓度相对较高,产品可能会出现浑浊。一旦稀释使用,溶液应澄清。

包装

所有20种氨基酸的1 mL水溶液。
该产品可以大包装形式供货,也可按需进行包装。 如需关于价格、供货形式及包装的信息,请联系稳定同位素客户服务

分析说明

氨基酸浓度因批次而异。请参见特定批次检验报告。

近似浓度(mM):
ASP 60
THR 35
SER 35
GLU 40
PRO 20
GLY 100
ALA 100
VAL 40
MET 10
ILE 30
LEU 45
TYR 10
PHE 16
HIS 5
LYS 15
ARG 10
GLN 20
ASN 20
TRP 20
CYS 20

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2 - Skin Irrit. 2

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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The ability to link soil microbial diversity to soil processes requires technologies that differentiate active microbes from extracellular DNA and dormant cells. Here, we use BONCAT (bioorthogonal non-canonical amino acid tagging) to measure translationally active cells in soils. We compare
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Caschera, F., Noireaux, V
Biotechniques, 58, 40-43 (2015)
Zhenzhen Liu et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1124, 72-81 (2019-06-10)
A simple and fast LC-MS/MS method was developed and validated for simultaneous quantification of 20 proteinogenic l-amino acids (AAs) in a small volume (5 μL) of mouse plasma. Chromatographic separation was achieved on an Intrada Amino Acid column within 13 min via
Filippo Caschera et al.
BioTechniques, 58(1), 40-43 (2015-01-22)
Here we present a procedure for preparing amino acid mixtures--having both the desired composition and a physiological pH--at high concentrations for cell-free expression systems. Up to 2.1 mg/mL of active protein was synthesized in batch mode reactions with an all
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