767964
Cell Free Amino Acid Mixture - 13C,15N
5-100 mM in water, 98 atom % 15N, 98 atom % 13C
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About This Item
UNSPSC Code:
12352209
NACRES:
NA.12
isotopic purity
98 atom % 13C
98 atom % 15N
Quality Level
form
solid
concentration
5-100 mM in water
technique(s)
bio NMR: suitable
protein expression: suitable
shipped in
dry ice
storage temp.
−20°C
Related Categories
Application
This product suitable as source of Stable Isotope Labeled amino acids for Cell Free Protein Synthesis. For Mass Spec applications, consider product 909653, Stable Isotope Labeled Amino Acid Mixture for Mass Spec.
The product may appear turbid, due to the relatively high concentration of tyrosine. Once diluted for use, solution should be clear.
The product may appear turbid, due to the relatively high concentration of tyrosine. Once diluted for use, solution should be clear.
Packaging
1 mL aqueous solution of all 20 amino acids.
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.
Analysis Note
Amino acid concentrations vary by lot. Please refer to the specific lot Certificate of Analysis.
Approximate concentrations (mM):
ASP 60
THR 35
SER 35
GLU 40
PRO 20
GLY 100
ALA 100
VAL 40
MET 10
ILE 30
LEU 45
TYR 10
PHE 16
HIS 5
LYS 15
ARG 10
GLN 20
ASN 20
TRP 20
CYS 20
Approximate concentrations (mM):
ASP 60
THR 35
SER 35
GLU 40
PRO 20
GLY 100
ALA 100
VAL 40
MET 10
ILE 30
LEU 45
TYR 10
PHE 16
HIS 5
LYS 15
ARG 10
GLN 20
ASN 20
TRP 20
CYS 20
required but not provided
Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Estelle Couradeau et al.
Nature communications, 10(1), 2770-2770 (2019-06-27)
The ability to link soil microbial diversity to soil processes requires technologies that differentiate active microbes from extracellular DNA and dormant cells. Here, we use BONCAT (bioorthogonal non-canonical amino acid tagging) to measure translationally active cells in soils. We compare
Zhenzhen Liu et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1124, 72-81 (2019-06-10)
A simple and fast LC-MS/MS method was developed and validated for simultaneous quantification of 20 proteinogenic l-amino acids (AAs) in a small volume (5 μL) of mouse plasma. Chromatographic separation was achieved on an Intrada Amino Acid column within 13 min via
Preparation of amino acid mixtures for cell-free expression systems
Caschera, F., Noireaux, V
Biotechniques, 58, 40-43 (2015)
Sara Calhoun et al.
Communications biology, 4(1), 333-333 (2021-03-14)
Microalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus
Filippo Caschera et al.
BioTechniques, 58(1), 40-43 (2015-01-22)
Here we present a procedure for preparing amino acid mixtures--having both the desired composition and a physiological pH--at high concentrations for cell-free expression systems. Up to 2.1 mg/mL of active protein was synthesized in batch mode reactions with an all
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