ZRB2373
Anti-Laminin-5 Antibody, clone 1D3 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
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所有图片(10)
CSF 140 kDa subunit, Cell-scattering factor 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin subunit gamma-2, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma
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生物来源
rabbit
质量水平
重组
expressed in HEK 293 cells
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
1D3, recombinant monoclonal
描述
1D3 Clone
产品线
ZooMAb® learn more
形式
lyophilized
分子量
calculated mol wt 130.98 kDa
observed mol wt ~140 kDa
纯化方式
using Protein A
种属反应性
human
种属反应性(根据同源性预测)
monkey
包装
antibody small pack of 25 μL
环保替代产品特性
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
技术
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG
表位序列
C-terminal half
Protein ID登记号
UniProt登记号
环保替代产品分类
运输
ambient
储存温度
2-8°C
基因信息
human ... LAMC2(3918)
一般描述
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特异性
Clone 1D3 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Laminin-5. It targets an epitope within 24 amino acids from the C-terminal half.
免疫原
KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal half of human Laminin subunit 2 (Laminin-5).
应用
Quality Control Testing
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Laminin-5 in A431 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Laminin-5 in A431 cell membrane extract.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Laminin-5 in Human placenta tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Laminin-5 in A431 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Laminin-5 in A431 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Laminin-5 in A431 cell membrane extract.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Laminin-5 in Human placenta tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Laminin-5 in A431 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
目标描述
Laminin subunit gamma-2 (UniProt: Q13753; also known as Cell-scattering factor 140 kDa subunit, CSF 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma) is encoded by the LAMC2 (also known as LAMB2T, LAMNB2) gene (Gene ID: 3918) in human. Laminin is a major basement membrane glycoprotein consisting of α, β, and γ chains that are bound to each other by disulfide bonds. It is a key component of the anchoring complex connecting keratinocytes to the underlying dermis. Over 15 different isoforms have been reported in the laminin family that are expressed in a tissue-specific manner and play different role in each tissue. γ2, a subunit of Laminin-5 (also known as Laminin-332), is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form. It is reported to be frequently over-expressed as a monomer or as the β3-γ2 heterodimer in invasive cancers and is regarded as an invasion marker. It has 8 EGF-like domains. The cell adhesion and motility activities of Laminin-5 are reported to be regulated by the proteolytic cleavage of the short arm of Laminin 2 chain by BMP-1 or MT1-MMP. This cleavage releases a 45 kDa N-terminal proteolytic fragment (γ2pf). The short arm and γ2pf can be further cleaved by serine proteases and by matrix metalloproteinases resulting in the release of small N-terminal fragments. γ2pf‐like fragments have been reported to be present in conditioned media of cultured cancer cells and in sera from human cancer subjects. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Miyazaki, K., et al. (2016). Cancer Sci. 107(12); 1909-1918; Sato, H., et al. (2014). Cancer Sci. 105(2); 168-175; Schneider, H., et al. (2007). Eur. J. Cell Biol. 86(11-12); 701-717).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
重悬
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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