biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6B4
monoclonal
form
liquid
mol wt
220 kDa
species reactivity
mouse, human
concentration
1.36 mg/mL
technique(s)
ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1:500-1:3000
isotype
IgG1
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... BRCA1(672)
Application
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC, IP, ChIP. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Biochem/physiol Actions
BRCA1-6B4 recognizes full length BRCA1, a 220 kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51. BRCA1 is predominantly expressed in proliferating and differentiating cells and contributes to cellular growth.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Features and Benefits
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General description
The gene BRCA1 is mapped to human chromosome 17q21 and codes for a protein with zinc finger motif and a set of acidic residues. BRCA1 is localized to the nucleus and cytoplasm.
Immunogen
BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.
Physical form
Phosphate-buffered saline, no preservative added.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Location of BRCA1 in human breast and ovarian cancer cells.
Scully R, et al.
Science, 272(5258), 123-123 (1996)
Epidemiology of breast cancer.
Key T J, et al.
Lancet Oncology, 2(3), 133-140 (2001)
The nuclear localization sequences of the BRCA1 protein interact with the importin-α subunit of the nuclear transport signal receptor.
Chen C F, et al.
The Journal of Biological Chemistry, 271(51), 32863-32868 (1996)
Anuratha Sakthianandeswaren et al.
Cancer discovery, 8(8), 988-1005 (2018-06-09)
ADP-ribosylation is an important posttranslational protein modification that regulates diverse biological processes, controlled by dedicated transferases and hydrolases. Here, we show that frequent deletions (∼30%) of the MACROD2 mono-ADP-ribosylhydrolase locus in human colorectal cancer cause impaired PARP1 transferase activity in
Michal Franek et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 64(11), 669-686 (2016-09-30)
DNA damage response (DDR) in ribosomal genes and mechanisms of DNA repair in embryonic stem cells (ESCs) are less explored nuclear events. DDR in ESCs should be unique due to their high proliferation rate, expression of pluripotency factors, and specific
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