RIP
Imprint® RNA免疫沉淀试剂盒
High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA
别名:
Magnetic Bead RNA Immunoprecipitation, RNA Immunopreciptation, mRNA Immunopreciptation, microRNA Immunoprecipitation
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关于此项目
NACRES:
NA.54
UNSPSC Code:
41116158
Quality Level
General description
RNA免疫沉淀（RIP）是研究核糖核蛋白（RNP）复合物中RNA结合蛋白（RBP）及其RNA靶点的强大方法。抗特定RBP的抗体被用于共沉淀RNP，即RBP及其RNA伴侣。然后，可以通过下一代测序鉴定RNA或通过RT-PCR检测特定RNA。
Application
Imprint® RNA免疫沉淀试剂盒已可用于：
- 适用于下游应用
- 从个体靶标表征到全基因组图谱分析技术
- 信号转导途径的表征
- 验证ChIp-chIP和ChIP-seq数据
Features and Benefits
- 更高结合能力的Xtra蛋白A磁珠
- 细胞裂解和免疫沉淀的分步实验方案
- 包含RNA酶抑制剂和无RNA酶试剂，以保持RNA的完整性
- 内附阴性对照，且包含抗体
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书 & 价格
- IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
- Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
存储类别
3 - Flammable liquids
wgk
WGK 3
flash_point_f
100.4 °F
flash_point_c
38 °C
signalword
Danger
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
法规信息
低风险生物材料
常规特殊物品
此项目有
实验方案
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
使用RIP试剂盒进行哺乳动物细胞Ago抗体-RNA免疫沉淀的实验方案和流程
商品
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Sigma Imprint RNA 免疫沉淀试剂盒用于从 HeLa 细胞中共纯化人 argonaute 2 (Ago2) 相关 RNA。MicroRNA通过Mysticq 试剂进行逆转录和定量。
相关内容
Instructions
Yiwei Liao et al.
Journal of cellular biochemistry, 118(7), 1889-1899 (2017-01-26)
Temozolomide (TMZ)-based chemotherapy is a standard strategy for glioma, while chemoresistance remains a major therapeutic challenge. Recent evidence highlights the crucial regulatory roles of long non-coding RNAs (lncRNA) in tumor biology. However, the roles and regulatory mechanisms of lncRNA cancer
Qiyuan Wang et al.
Journal of cellular biochemistry, 118(11), 3775-3784 (2017-04-06)
Osteoarthritis (OA) is characterized by progressive destruction of articular cartilage, resulting in significant disability. Inflammatory cytokines commonly initiate the extreme changes in the synovium and cartilage microenvironment of the OA patients, subsequently resulting in cell dysfunctions, especially synoviocyte dysfunction. We
Lin Cao et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 93, 570-579 (2017-07-08)
Non-small cell lung cancer (NSCLC), known as the most common type of lung cancer, has caused great economic losses and brought the patients with NSCLC great suffering. The NSCLC chemo-resistance to cisplatin (DDP) -based chemotherapy remains a huge challenge. The
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