RIP
Imprint® RNA免疫沉淀试剂盒
High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA
别名:
Magnetic Bead RNA Immunoprecipitation, RNA Immunopreciptation, mRNA Immunopreciptation, microRNA Immunoprecipitation
Application
Imprint® RNA免疫沉淀试剂盒已可用于：
- 适用于下游应用
- 从个体靶标表征到全基因组图谱分析技术
- 信号转导途径的表征
- 验证ChIp-chIP和ChIP-seq数据
Features and Benefits
- 更高结合能力的Xtra蛋白A磁珠
- 细胞裂解和免疫沉淀的分步实验方案
- 包含RNA酶抑制剂和无RNA酶试剂，以保持RNA的完整性
- 内附阴性对照，且包含抗体
General description
RNA免疫沉淀（RIP）是研究核糖核蛋白（RNP）复合物中RNA结合蛋白（RBP）及其RNA靶点的强大方法。抗特定RBP的抗体被用于共沉淀RNP，即RBP及其RNA伴侣。然后，可以通过下一代测序鉴定RNA或通过RT-PCR检测特定RNA。
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder
- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
- R1158Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μL
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
存储类别
3 - Flammable liquids
wgk
WGK 3
flash_point_f
100.4 °F
flash_point_c
38 °C
法规信息
低风险生物材料
常规特殊物品
此项目有
Alex Michael Ward et al.
Scientific reports, 6, 34379-34379 (2016-09-30)
Dengue virus (DENV) is a mosquito-borne Flavivirus classified into four serotypes (DENV-1-4) that causes Dengue fever (DF), Dengue hemorrhagic Fever (DHF) or Dengue shock syndrome (DSS). An estimated 390 million people are at risk for infection with DENV and there
Shuai Xiao et al.
DNA and cell biology, 37(2), 117-125 (2018-02-08)
P73 antisense RNA 1T (non-protein coding), known as TP73-AS1 or PDAM, is a long noncoding RNA (lncRNA), which may regulate apoptosis by regulation of p53-dependent antiapoptotic genes. An abnormal change of TP73-AS1 expression was noticed in cancers. The effects of
Long non-coding RNA HOTAIR is targeted and regulated by miR-141 in human cancer cells.
Chiyomaru T, et al.
The Journal of Biological Chemistry, 289(19), 12550-12565 (2014)
Qingling Yuan et al.
The international journal of biochemistry & cell biology, 98, 1-9 (2018-02-24)
Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy. Besides, increasing evidence has demonstrated that long non-coding RNA (lncRNA) HOTTIP played a crucial role in cancer pathogenesis. MiR-637-mediated Akt1 was involved in cell growth, invasion and migration in various
Zhi-Jun Zhu et al.
Biochemical and biophysical research communications, 497(4), 971-977 (2018-02-11)
Long non-coding RNAs are critically involved in oncogenesis in various cancer types. Here we reported a novel lncRNA signature correlated with progression of non-small cell lung cancer (NSCLC). In particular, we identified elevated expression of terminal differentiation-induced noncoding RNA (TINCR)
商品
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Sigma Imprint RNA 免疫沉淀试剂盒用于从 HeLa 细胞中共纯化人 argonaute 2 (Ago2) 相关 RNA。MicroRNA通过Mysticq 试剂进行逆转录和定量。
实验方案
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
使用RIP试剂盒进行哺乳动物细胞Ago抗体-RNA免疫沉淀的实验方案和流程
相关内容
Instructions
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