form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
sodium pyruvate: 0.22 g/L
HEPES: no
L-glutamine: yes
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
General description
营养混合物Ham#8242;s F-12由Ham开发，其含有较高水平的氨基酸、维生素和微量元素。其将腐胺和亚油酸加入配方中。该营养培养基最初设计用于中国仓鼠卵巢和肺细胞的无血清生长。当与全血或透析血清一起使用或与激素和转铁蛋白组合使用时，它可广泛用于哺乳动物和杂交瘤细胞的生长。
Application
营养混合物F-12 Ham可用于以下应用：
Kaighn#8242;s 改良的Ham#8242;s F-12和Coon#8242;s F-12增加了氨基酸和丙酮酸，以及改性盐（Konigsbergs）的浓度。该培养基被设计用于支持分化的大鼠和鸡细胞以及原代人肝细胞的生长。
- HeLa（人子宫颈腺癌细胞）细胞在补充有10％热灭活胎牛血清的该培养基（营养混合物F-12 Ham）进行中生长
- 在维持和培养期间用于不同细胞的细胞培养（在营养混合物F-12 HAM培养基与其他组分中，A549细胞系保持为单层）
- 用作Cowdria ruminantium繁殖的培养基
- 用于制备初级混合胶质细胞培养物
Kaighn#8242;s 改良的Ham#8242;s F-12和Coon#8242;s F-12增加了氨基酸和丙酮酸，以及改性盐（Konigsbergs）的浓度。该培养基被设计用于支持分化的大鼠和鸡细胞以及原代人肝细胞的生长。
Preparation Note
并补充2.5 g/L碳酸氢钠。
配方中每升培养基含有11.1克的粉末。
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| N3520-10X1L | 04061834116172 |
| N3520-10L | 04061834116165 |