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L3771

十二烷基硫酸钠

Name: 十二烷基硫酸钠
Brand: SIGMA

BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

别名:

SDS, 十二烷基硫酸盐钠盐, 十二烷基硫酸钠, 月桂基硫酸盐钠盐, 月桂基硫酸钠

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About This Item

线性分子式:

CH₃(CH₂)₁₁OSO₃Na

CAS号:

151-21-3

分子量:

288.38

Beilstein:

3599286

EC 号:

205-788-1

MDL编号:

MFCD00036175

UNSPSC代码:

12161902

eCl@ss:

39093306

PubChem化学物质编号:

24896351

NACRES:

NB.22

生物来源

synthetic

质量水平

200

等级

for molecular biology

描述

anionic

产品线

BioReagent

检测方案

≥98.5% (GC)

形式

powder

分子量

micellar 18,000

聚集数

技术

electrophoresis: suitable

杂质

≤2.0% water (Karl Fischer)

CMC

7-10 mM (20-25°C)

mp

204-207 °C (lit.)

转变温度

cloud point >100 °C

溶解性

water: soluble

密度

1.03 g/cm³ at 20 °C (68 °F)

痕量阴离子

chloride (Cl^-): ≤500 ppm
phosphate (PO₄^3-): ≤10 ppm

痕量阳离子

heavy metals (as Pb): ≤10 ppm

吸光度

≤0.1 at 260 at 3%
≤0.1 at 280 at 3%

HLB

适用性

suitable for electrophoresis

异质活性

DNase, RNase, none detected

储存温度

room temp

SMILES字符串

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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一般描述

十二烷基硫酸钠（SDS）也称月桂基硫酸钠，它是一种阴离子表面活性剂，广泛应用于各种科学应用，特别是分子生物学、生物化学和细胞生物学研究。它的功效来源于两性分子特性，这种特性使其可以同时与疏水和亲水分子相互作用。SDS是一种可以使蛋白质增溶和变性的多功能去污剂，这种特性使其成为蛋白质提取和纯化实验中的基本工具。在SDS-PAGE电泳中，通过破坏蛋白质结构，SDS有助于根据分子量进行蛋白质分离。

除了蛋白质分析以外，SDS的应用还包括脂质的电泳分离，从而使研究人员能够对这些重要的细胞成分进行研究和表征。此外，各种杂交和分子生物学技术中也用到了SDS，这体现了它在各种研究领域中的多功能性。SDS在离子和非离子条件下的功效进一步扩展了其用途，使其适用于各种实验环境和要求。它能够通过结合肽链并产生负电荷来裂解蛋白，从而有效消除了凝胶分离中蛋白质形状这一影响因素，确保了准确的蛋白质尺寸测定。

应用

十二烷基硫酸钠已用于：

染色体免疫沉淀
SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）
作为用于免疫印迹的放射免疫沉淀检测缓冲液的一种组分

用于增溶和蛋白质变性，适用于PAGE变性应用。大多数蛋白质以1.4 g SDS/g 蛋白质的比例结合SDS。相对于结合的SDS自带的总负电荷，蛋白质固有电荷几乎可忽略不计。每种蛋白质的质荷比基本相同，且基于分子大小在凝胶中迁移。

阴离子洗涤剂

特点和优势

用于分子生物学和生物化学研究的多功能表面活性剂
适用于电泳应用
经测试证实重金属污染浓度低，从而确保其适用于各种应用。

分析说明

针对用于变性聚丙烯酰胺凝胶电泳进行了测试。

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

如需了解生化试剂系列的更多信息，请填写此表。

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说明

价格

警示用语：

Danger

危险声明

H228,H302 + H332,H315,H318,H335,H412

预防措施声明

P210 - P273 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338

危险分类

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 2

闪点(°F)

338.0 °F

闪点(°C)

170 °C

个人防护装备

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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