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HSANGERV

桑格全基因组 慢病毒 CRISPR 文库

Human, Virus Format

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关于此项目

UNSPSC Code:
41105904
Concentration:
1x106  VP/ml (via p24 assay)

Quality Level

200

packaging

pkg of 10 μL (384-well plate)

concentration

1x106  VP/ml (via p24 assay)

application(s)

CRISPR

shipped in

dry ice

storage temp.

−70°C

General description

两名基因组编辑领导者Sigma-Aldrich和Wellcome Trust Sanger Institute联手打造了第一个阵列化的慢病毒CRISPR基因敲除文库。基于文献中已验证的技术，Sanger CRISPR库将使您的实验室走在竞争的最前沿，以实现下一个重大发现。

Application

功能基因组学/筛选/靶标验证

Features and Benefits

  • Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
  • Simplify the workflow with puromycin selection
  • Illuminate CRISPR-expressing cells with BFP

Additional Features
  • Better, not bigger: Two optimized clones per gene reduces the time, cost, and scale of screening experiments
  • Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
  • Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

Packaging

384-well plates

Physical form

10 μl of lentivirus at ≥106 particles/ml in 102×384-well plates

Other Notes

Additional libraries are available:
Human, Glycerol Format
Mouse, Glycerol Format
Mouse, Virus Format
Lentivirus Transduction Particles of gRNA-only lenti-based vector. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones.


Request a Quote or More Information
This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

Legal Information

CRISPR Use License Agreement

Lentiviral License Agreement

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存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

低风险生物材料
高风险级别生物产品--病毒，疫苗等

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

Sanger Sequencing Steps & Method

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

桑格测序步骤与方法

了解桑格测序步骤或链终止法、DNA 测序的工作原理以及如何在研究中准确读取桑格测序结果。

商品

Sanger Whole Genome CRISPR Libraries

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

The Researcher’s Guide to Designing CRISPR and RNAi Screening Experiments

This screening guide covers how to choose a cell line, a screening library, and experimental conditions as well as tips for designing and performing your experiment.

查看所有文章
Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.
Metzakopian, E. et al.
Scientific Reports, 7 (2017)

全球贸易项目编号

货号GTIN
HSANGERV-1EA04061826266205