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安全信息

MSANGERV

Sigma-Aldrich

桑格全基因组 慢病毒 CRISPR 文库

Mouse, Virus Format

别名:

Arrayed CRISPR library, Sanger CRISPR library

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About This Item

UNSPSC代码:
41105904

质量水平

200

包装

pkg of 10 μL (384-well plate)

浓度

1x106  VP/ml (via p24 assay)

应用

CRISPR

运输

dry ice

储存温度

−70°C

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相关类别

一般描述

两名基因组编辑领导者Sigma-Aldrich和Wellcome Trust Sanger Institute联手打造了第一个阵列化的慢病毒CRISPR基因敲除文库。基于文献中已验证的技术，Sanger CRISPR库将使您的实验室走在竞争的最前沿，以实现下一个重大发现。

应用

功能基因组学/筛选/靶标验证

特点和优势

  • Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
  • Simplify the workflow with puromycin selection
  • Illuminate CRISPR-expressing cells with BFP

Additional Features
  • Better, not bigger: Two optimized clones per mouse gene reduces the time, cost, and scale of screening experiments
  • Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
  • Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

包装

384 well plates

组分

Lentivirus Transduction Particles of gRNA-only lenti-based vectors. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones


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外形

10μl of lentivirus at ≥106 particles/ml in ~120×384 well plates

其他说明

This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

推荐产品

Additional libraries are available:
Human, Glycerol Format
Mouse, Glycerol Format
Human, Virus Format

法律信息

CRISPR Use License Agreement

Lentiviral License Agreement

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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访问文档库

Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.
Metzakopian, E. et al
Scientific Reports, 7 (2017)

商品

Sanger Whole Genome CRISPR Libraries

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

Sanger全基因组CRISPR芯片文库

全基因组功能缺失筛查是发现生物过程背后的基因和途径的有效方法。现在，每个基因都可通过两个优化的 gRNA 完全敲除。通过最大限度减少克隆数量，可确保尽可能特异性的筛选，同时控制时间和成本。

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

利用慢病毒成功转导

获取处理慢病毒、优化实验设置、测定慢病毒颗粒滴度以及选择实用转导产品方面的贴士。

实验方案

Sanger Sequencing Steps & Method

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

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