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Merck
CN
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文件

FCANOS1

Sigma-Aldrich

一氧化氮合酶检测系统，荧光法

sufficient for 200 reactions

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UNSPSC代码:
12352303
NACRES:
NA.32

用途

sufficient for 200 reactions

质量水平

200

存货情况

not available in Japan

运输

wet ice

储存温度

2-8°C

基因信息

human ... NOS1(4842) , NOS2(4843) , NOS2B(201288) , NOS2C(645740) , NOS3(4846)

生化/生理作用

该系统可简单特异地检测生理条件下活细胞内游离一氧化氮(NO)和一氧化氮合成酶(NOS)的活性。一氧化氮由L-精氨酸经NOS作用而形成，是一种独特的细胞信号分子，可同时作为细胞内和细胞外的信使分子。NO与血管舒张、神经传递、细胞毒性和炎症都有一定关联。
一氧化氮合酶活性的标准检测方法针对的是不含细胞的可溶性酶制品，如细胞裂解物、组织粗提物或纯化酶。该方法可检测由放射性标记的精氨酸底物转换而成的放射性瓜氨酸的产量（Sigma货号NOS-1）。与之不同的是，本品是基于荧光的细胞相关NOS检测系统，通过非放射性方法检测细胞内NO产量。 采用可穿过细胞膜的二乙酸盐形式的二氨基荧光素(DAF-2 DA)替代放射性同位素。DAF-2 DA可迅速穿入细胞，进入细胞后被细胞内酯酶水解成为DAF-2，DAF-2与经NOS作用产生的NO反应，生成带有荧光的三唑荧光素。随后，可在激发波长492 nm 和发射波长515 nm 下，对该荧光性产物进行定量检测。

法律信息

DAF-2 DA由Daiichi Pure Chemicals有限公司生产。
美国专利号5,874, 590

仅试剂盒组分

产品编号
说明
  • Diphenyleneiodonium Chloride (DPI) 1 mM 200 μL
  • Arginine Substrate Solution for NOS Detection, Fluorimetric 2 mL

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • D225Diaminofluorescein-2 diacetate (DAF2-DA) 1 mL/vial化学品安全说明书
  • N9910β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt, vial of 0.30-0.36 mg 1 vial化学品安全说明书

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Zeming Zhang et al.
Journal of cellular physiology, 234(11), 19715-19727 (2019-04-16)
Pulmonary arterial hypertension (PAH) is characterized by pulmonary vascular remodeling of the precapillary pulmonary arteries, with excessive proliferation of vascular cells. This study was performed to examine the effects of long noncoding RNA CPS1 intronic transcript 1 (CPS1-IT) on PAH
Sonia Agrawal et al.
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The development of the latent phenotype of Mycobacterium tuberculosis (Mtb) in the human lungs is the major hurdle to eradicate Tuberculosis. We recently reported that exposure to nitrite (10 mM) for six days under in vitro aerobic conditions completely transforms the
D J Stuehr
Annual review of pharmacology and toxicology, 37, 339-359 (1997-01-01)
Research on the biological roles of nitric oxide has revealed that it functions as an important signal and effector molecule in a variety of physiologic and pathologic settings. In animals, nitric oxide is synthesized enzymatically from L-arginine through the actions
D A Geller et al.
Cancer metastasis reviews, 17(1), 7-23 (1998-04-17)
Nitric oxide (NO) is a potent biologic mediator with diverse physiologic and pathophysiologic roles. NO is produced from L-arginine by the family of nitric oxide synthase (NOS) enzymes, forming the free radical NO and citrulline as byproduct. Three distinct isoforms
H Kojima et al.
Chemical & pharmaceutical bulletin, 46(2), 373-375 (1998-03-21)
Endogenous nitric oxide (NO) appears to modulate many physiological and pathophysiological processes. In order to obtain direct evidence for NO functions in vivo, we have developed 4,5-diaminofluorescein (DAF-2) as a novel fluorescent indicator for NO. Green-fluorescent triazolofluorescein formed by the
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