产品名称
营养混合物 F-12 Ham, powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, Coon′s Modification, suitable for cell culture
表单
powder
技术
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
组分
phenol red: 0.00125 g/L
L-glutamine: 0.292 g/L
sodium pyruvate: 0.22 g/L
glucose: 1.802 g/L
运输
ambient
储存温度
2-8°C
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应用
库恩对哈姆氏 F-12 的改造是为培养通过病毒融合产生的杂交细胞而开发的。修饰包括使氨基酸和丙酮酸加倍,并包括抗坏血酸。盐浓度也发生了改变。配方中含有 0.863 mg/L 硫酸锌,这可能使其不适合培养小鼠 L -细胞。
制备说明
按照每升培养基11.5克粉末配制。
补充2.68 g/L碳酸氢钠。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物来源培养基
此项目有
Xiaojiao Xue et al.
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cAMP is a crucial mediator of multiple cell signaling pathways. This cyclic nucleotide requires strict spatiotemporal control for effective function. Light-activated proteins have become a powerful tool to study signaling kinetics due to having quick on/off rates and minimal off-target
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性,应注意防潮。
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