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Merck
CN

D9785

DMEM/F-12

With HEPES, without ʟ-glutamine, ʟ-leucine, ʟ-lysine, ʟ-methionine, CaCl2, MgCl₂, MgSO4, sodium bicarbonate, and phenol red, powde, suitable for cell cutlure

别名:

杜氏改良Eagle 培养基/营养混合物 F-12 Ham, DME/F-12, 1:1混合

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217417

主要文件

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产品名称

DMEM/营养混合物 F-12 Ham, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
NaHCO3: no
phenol red: no
glucose: 3.15 g/L

shipped in

ambient

storage temp.

2-8°C

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Preparation Note

配制成每升培养基含 14.8 克粉末。
补充 0.1545 g/L 氯化钙，0.365 g/L L -谷氨酰胺，0.05905 g/L L -亮氨酸，0.09125 g/L L -赖氨酸，0.0612 g/L 氯化镁，0.04884 g/L 硫酸镁，0.0172 g/L L -蛋氨酸，1.2 g/L 碳酸氢钠。

General description

提供的最基本的制剂。该制剂由希望从 (1;1) DME/Ham F-12 的必需组分开始，并具有为自身应用优化制剂的灵活性的研究者使用。该基础制剂特别适用于标记氨基酸的细胞蛋白代谢研究。

存储类别

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

动植物来源培养基
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
25D152
25D152
0000278759
0000264744
0000278759

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Yuuma Furutani et al.
Cell biochemistry and function, 29(7), 577-581 (2011-08-23)
Magnesium (Mg) deficiency induces the production of free radicals, increases cytosolic ionized calcium concentration, and modulates the function of skeletal muscle in rats. The present study examined the effects of Mg deficiency on the gene expression of molecules related to
Vincent J Munster et al.
Nature, 585(7824), 268-272 (2020-05-13)
An outbreak of coronavirus disease 2019 (COVID-19), which is caused by a novel coronavirus (named SARS-CoV-2) and has a case fatality rate of approximately 2%, started in Wuhan (China) in December 20191,2. Following an unprecedented global spread3, the World Health Organization
Wei Ye et al.
Frontiers in microbiology, 11, 1105-1105 (2020-07-01)
Human enteroviruses are responsible for diverse diseases, from mild respiratory symptoms to fatal neurological complications. Currently, no registered antivirals have been approved for clinical therapy. Thus, a therapeutic agent for the enterovirus-related disease is urgently needed. Remdesivir (GS-5734) is a
Rui Yan et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 103, 1490-1497 (2018-06-06)
Neonatal brain hypoxia is a disease that affects the nervous system in children. Salidroside is a compound that has an anti-hypoxic effect, but the mechanism of salidroside in neonatal cerebral hypoxia is unclear. Hence, we investigated the regulatory effect and
Huntingtin promotes mTORC1 signaling in the pathogenesis of Huntington's disease.
Pryor WM, Biagioli M, Shahani N, et al.
Science Signaling, 7(349), ra103-ra103 (2014)
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商品

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

钙在细胞培养中的应用

钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。

实验方案

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

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