推荐产品
生物来源
bovine pancreas
质量水平
类型
Type IV
表单
lyophilized powder
比活
≥2,000 Kunitz units/mg protein
分子量
~31 kDa
纯化方式
chromatography
组成
Protein, ≥80%
技术
DNA purification: suitable
溶解性
0.15 M NaCl: soluble 5.0 mg/mL, clear, colorless
适用性
suitable for molecular biology
应用
diagnostic assay manufacturing
diagnostic assay manufacturing
异质活性
Chymotrypsin ≤0.5%
Protease ≤0.05%
RNase ≤0.02%
运输
wet ice
储存温度
−20°C
正在寻找类似产品? 访问 产品对比指南
相关类别
应用
生化/生理作用
单位定义
外形
制备说明
分析说明
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
从最新的版本中选择一种：
分析证书(COA)
How should I prepare a solution of Product D5025, DNAse I?
This enzyme can be reconstituted in 0.15 M NaCl at a concentration of 10 mg/ml.
Are stock solutions of Product D5025, Deoxyribonuclease I (DNase I), stable?
Solutions of DNAse I (10 mg/ml) in 0.15 M NaCl may lose 10% of its activity when stored for a week in aliquots at -20°C. The same solutions stored in aliquots at 2 - 8°C. lose approximately 20% activity.
How do Deoxyribonuclease I (DNase I), products D5025 and D4527 differ from each other?
Both of these products have the same specific activity (>2000 units/mg protein) for Dnase I. The enzyme impurity levels for each, however, are different. D5025 has the following impurity specification: Chymotrypsin (<0.5%), Protease (<0.05%), and RNase (<0.02%). The impurity specifications for D4527 are: Chymotrypsin (<0.01%), Protease (<0.005%), and RNase (<0.01%).
How can I convert units of Product D5025, Deoxyribonuclease I (DNase I), to mass?
The mg of solid for each unit package size will be indicated on the label of the package. For a particular lot, you can calculate the specific activity in units per mg solid by multiplying the specific activity in units per mg protein by the percent protein divided by 100.
What concentration of Product D5025, Deoxyribonuclease I (DNase I), is required to remove DNA from solutions?
DNA can be removed from preparations by incubation with 20 - 50 ug DNAse I in the presence of 50 mM Tris-HCl buffer, pH 7.5, 10 mM magnesium chloride, at 37°C. for 60 min.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
实验方案
To standardize a procedure for the enzymatic assay of Deoxyribonuclease I.
建立脱氧核糖核酸酶I酶促测定的标准化操作流程。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门