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CASP3C

Caspase 3 检测试剂盒（比色法）

Name: Caspase 3 检测试剂盒（比色法）
Brand: SIGMA

别名:

胱天蛋白酶3(Caspase-3)比色法活性检测试剂盒

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About This Item

UNSPSC代码:

12161503

NACRES:

NA.84

该产品已停产。建议选择以下替代产品：

Slide 1 of 2

Sigma-Aldrich

A2058

牛血清白蛋白来源于牛血清

lyophilized powder, essentially IgG-free, low endotoxin, BioReagent, suitable for cell culture

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与停产产品比较

$牛血清白蛋白来源于牛血清 cold ethanol fraction, pH 5.2, ≥96%$

Sigma-Aldrich

A4503

牛血清白蛋白来源于牛血清

cold ethanol fraction, pH 5.2, ≥96%

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此商品	A3912	A8022	A9647
$牛血清白蛋白来源于牛血清 cold ethanol fraction, pH 5.2, ≥96%$ A4503 牛血清白蛋白来源于牛血清	$牛血清白蛋白来源于牛血清 heat shock fraction, pH 5.2, ≥96%$ A3912 牛血清白蛋白来源于牛血清	$牛血清白蛋白来源于牛血清 heat shock fraction, pH 5.2, ≥96%$ A8022 牛血清白蛋白来源于牛血清	$牛血清白蛋白来源于牛血清 heat shock fraction, pH 7, ≥98%$ A9647 牛血清白蛋白来源于牛血清
assay ≥96%	assay ≥96%	assay ≥96%	assay ≥98%
biological source bovine	biological source bovine	biological source bovine	biological source bovine
origin USA origin	origin USA origin	origin USA origin	origin USA origin
Quality Level 300	Quality Level 200	Quality Level 300	Quality Level 300
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder

一般描述

Caspase 3 检测试剂盒（比色法）可在细胞溶解产物和半胱天冬酶3纯化制备物样本中快速、有效地检测半胱天冬酶3的活性。

Caspase 3 检测试剂盒（比色法）的检测原理为Caspase 3可水解乙酰基- Asp-Glu-Val-Asp对硝基苯胺（Ac-DEVD-pNA），释放对硝基苯胺（pNA）部分。然后在405 nm处测定对硝基苯胺(ε_mM=10.5)。再根据405 nm处的吸光度值，就可以计算出底物释放的pDNA浓度。此外，也可以将获得的校准曲线与pNA标准品曲线（试剂盒中包含pNA标准品）进行比较，计算出底物释放的pDNA浓度。

应用

该检测既可采用(a)1 mL的检测体系由分光度计来测定；也可采用(b)100 µL检测体系在96孔板中由ELISA酶标仪来测量。

单位定义

在pH 7.4，25 °C条件下，1单位的Caspase 3每分钟裂解1.0 μmol的Ac-DEVD-pNA。

其他说明

仅限使用17 megohm 水

仅试剂盒组分

产品编号

说明

Water (17-18 megaOhm) 125 mL

试剂盒组分也可单独购买

产品编号

说明

化学品安全说明书

A0835Ac-DEVD-CHO inhibitor .5 mg化学品安全说明书

A2559Ac-DEVD-pNA substrate 15 mg化学品安全说明书

p-Nitroaniline standard 1 mg

象形图

GHS06,GHS08

警示用语：

Danger

危险声明

H301 + H311 + H331,H373,H412

预防措施声明

P273 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P311 - P314

危险分类

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - STOT RE 2

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

闪点(°F)

415.4 °F - closed cup

闪点(°C)

213 °C - closed cup

法规信息

常规特殊物品

危险化学品

技术规格说明书

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

0000227425

0000183459

0000094631

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Caspase 3 检测试剂盒（比色法）

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仅试剂盒组分

试剂盒组分也可单独购买

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警示用语：

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