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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
DCS-31, monoclonal
分子量
antigen 33 kDa
包含
15 mM sodium azide
种属反应性
rat, mouse, human
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:1,000 using a cultured human tumor cell line extract
同位素/亚型
IgG2a
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... CDK4(1019)
mouse ... Cdk4(12567)
rat ... Cdk4(94201)
一般描述
Cdk4 exists, in part, as a multi-protein complex with a D-type cyclin, proliferating cell nuclear antigen (PCNA) and a protein inhibitor, p21Cip1. Cdk4 associates separately with p16, particularly in cells lacking a functional retinoblastoma protein.
Monoclonal Anti-Cdk4 (mouse IgG2a isotype) is derived from the DCS-31 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cyclin-dependent kinase 4 is localized in human chromosome 12q14.1.
特异性
The antibody reacts specifically with Cdk4 and does not recognize other Cdk types.
免疫原
recombinant human Cdk4 protein
应用
Monoclonal Anti-Cdk4 antibody was used in:
- immunoprecipitation of human melanoma cell line.
- immunofluorescence of fibrosarcoma cells.
- western blot analysis
- immunofluorescence
- immunohistochemistry
生化/生理作用
In association with cyclins, cyclin-dependent kinases (CDKs) forms active kinase complexes which is responsible for regulating cell cycle progression in eukaryotic cells. Within the complexes, the CDKs serves a catalytic protein kinase activity. This catalytic activity is regulated by two mechanisms: protein phosphorylation and association of regulatory subunits.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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商品
Quantitative and qualitative western blotting to validate knockdown by esiRNA.
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