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YEAST1

Sigma-Aldrich

酵母转化试剂盒

reagents for introducing plasmid DNA into yeast

别名:

lithium acetate yeast transformation

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.85

等级

for molecular biology

质量水平

用途

 kit sufficient for >100 standard transformations

技术

transformation: suitable

运输

dry ice

储存温度

−20°C

一般描述

Sigma 的酵母转化试剂盒包含了通过醋酸锂方法高效转化酵母的所有必要试剂和对照品。

应用

适用于任何酵母菌株的转化。 方便灵活灵敏,只需 10 ng 的 DNA 即可获得阳性转化子;最佳效率在 0.1-3μg 范围。

特点和优势

  • 简单易用
  • 只需 10 ng 质粒 DNA
  • 任何酵母菌株的灵活性
  • 足以进行超过 100 次标准转换

组分

酵母转化试剂盒包含:
  • 转化缓冲液;100 ml;100 mM 醋酸锂、10 mM Tris HCl (pH 7.6) 和 1 mM EDTA
  • 平板缓冲液;100 mL;40%PEG,100 mM 醋酸锂,10 mM Tris HCl,pH 7.5,1 mM EDTA
  • 鲑鱼试验的脱氧核糖核酸,10 mg/mL;2 x 1 mL
  • 携带 ura 基因的对照酵母质粒 DNA pRS316;10 μg
  • 不含尿嘧啶的酵母合成脱落培养基补充剂;1 g

原理

用补充突变基因的质粒转化使转化子在缺乏所需成分的培养基上生长。通过在缓冲醋酸锂溶液中孵育,使酵母细胞具有转化能力。然后在含聚乙二醇 (PEG) 的溶液中将细胞与转化 DNA 和载体 DNA 一起孵育进行转化。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1化学品安全说明书

  • Y1501Yeast Synthetic Drop-out Medium Supplements, without uracil 1 g化学品安全说明书

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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在文件库中查找您最近购买产品的文档。

访问文档库

Francesco Palma et al.
FEMS microbiology letters, 272(1), 114-119 (2007-06-22)
The TBF-1 is an 11.9-kDa fruiting body specific protein of the Ascomycetes hypogeous fungus Tuber borchii Vittad. found in aqueous extract and the hyphal cell wall. The tbf-1 gene codes a 12-amino acid N-terminal stretch not present in mature protein.
James R Petrie et al.
PloS one, 7(4), e35214-e35214 (2012-04-24)
Monoacylglycerol acyltransferases (MGATs) are predominantly associated with lipid absorption and resynthesis in the animal intestine where they catalyse the first step in the monoacylglycerol (MAG) pathway by acylating MAG to form diacylglycerol (DAG). Typical plant triacylglycerol (TAG) biosynthesis routes such
Miren Zumárraga et al.
Proteins, 71(1), 250-260 (2007-10-13)
The generation of diversity for directed protein evolution experiments shows an important bottleneck in the in vitro random mutagenesis protocols. Most of them are biased towards specific changes that eventually confer a predicted and conservative mutational spectrum, limiting the exploration
DMSO-enhanced whole cell yeast transformation.
J Hill et al.
Nucleic acids research, 19(20), 5791-5791 (1991-10-25)
A two-hybrid screen identifies an unconventional role for the intermediate filament peripherin in regulating the subcellular distribution of the SNAP25-interacting protein, SIP30.
Gentil BJ
Journal of Neurochemistry, 131(5), 588-601 (2014)

商品

转化是将外源DNA引入细胞,引起遗传变异或基因修饰的过程。1928年,Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

基因工程和克隆的发展为研究领域开辟了表达和分离异源蛋白质的许多可能性。技术上巨大进步使得能够大规模表达和分离重组蛋白。

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

实验方案

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

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