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94046

磷酸氢二钠 溶液

BioUltra, 0.5 M in H2O

别名:

磷酸氢二钠 溶液

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关于此项目

线性分子式:
Na2HPO4
化学文摘社编号:
7558-79-4
分子量:
141.96
UNSPSC Code:
12161700
eCl@ss:
38070211
PubChem Substance ID:
57653505
NACRES:
NA.25
MDL number:
MFCD00003496
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product line

BioUltra

Quality Level

100

form

liquid

concentration

0.5 M in H2O

impurities

insoluble matter, passes filter test, ≤0.001% total nitrogen (N)

pH

8.8-9.4 (25 °C, 0.5 M in H2O)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

density

1.064 g/mL at 20 °C

anion traces

chloride (Cl-): ≤10 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 0.019, λ: 280 nm Amax: 0.016

application(s)

diagnostic assay manufacturing

SMILES string

[Na+].[Na+].OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

Application

磷酸氢二钠溶液可用于制备多种磷酸盐基生物缓冲液，用于细胞和分子生物学应用。
磷酸氢二钠溶液已用于：
  • 在钙化培养基中添加Dulbecco′改良伊格尔培养基（DMEM），以诱导血管平滑肌细胞（VSMC）的钙化
  • 制备CaCl2和 MgCl2的原液，以合成磷酸钙（CPB）和磷酸镁（MPB）
  • 补充2xYT琼脂板以培养BL21 DE3星型细胞和BL21dLac Gold型细胞

存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCP5771
BCCN9411
BCCM4374
BCCL9937
BCCM2829

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实验方案

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

醇脱氢酶（EC 1.1.1.1）的酶学测定

在测定醇脱氢酶活性时，该测定使用β-烟酰胺腺嘌呤二核苷酸磷酸和在340nm处的连续分光光度法进行测定。

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全球贸易项目编号

货号GTIN
94046-500ML-F04061833283745
94046-100ML-F04061838170309