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Merck
CN

94046

Sigma-Aldrich

磷酸氢二钠 溶液

BioUltra, 0.5 M in H2O

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别名:
磷酸氢二钠 溶液
线性分子式:
Na2HPO4
CAS号:
分子量:
141.96
MDL编号:
UNSPSC代码:
12161700
eCl@ss:
38070211
PubChem化学物质编号:
NACRES:
NA.25

产品线

BioUltra

质量水平

形式

liquid

浓度

0.5 M in H2O

杂质

insoluble matter, passes filter test
≤0.001% total nitrogen (N)

pH值(酸碱度)

8.8-9.4 (25 °C, 0.5 M in H2O)

溶解性

H2O: 0.5 M at 20 °C, clear, colorless

密度

1.064 g/mL at 20 °C

痕量阴离子

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

痕量阳离子

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

0.5 M in H2O

紫外吸收

λ: 260 nm Amax: 0.019
λ: 280 nm Amax: 0.016

应用

diagnostic assay manufacturing

SMILES字符串

[Na+].[Na+].OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

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应用

磷酸氢二钠溶液可用于制备多种磷酸盐基生物缓冲液,用于细胞和分子生物学应用。
磷酸氢二钠溶液已用于:
  • 在钙化培养基中添加Dulbecco′改良伊格尔培养基(DMEM),以诱导血管平滑肌细胞(VSMC)的钙化
  • 制备CaCl2和 MgCl2的原液,以合成磷酸钙(CPB)和磷酸镁(MPB)
  • 补充2xYT琼脂板以培养BL21 DE3星型细胞和BL21dLac Gold型细胞

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


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实验方案

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

在测定醇脱氢酶活性时,该测定使用β-烟酰胺腺嘌呤二核苷酸磷酸和在340nm处的连续分光光度法进行测定。

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

在测定透明质酸酶活性时,使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下,一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

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