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94046

Sigma-Aldrich

磷酸氢二钠 溶液

BioUltra, 0.5 M in H2O

别名:

磷酸氢二钠 溶液

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About This Item

线性分子式:
Na2HPO4
CAS号:
7558-79-4
分子量:
141.96
MDL编号:
MFCD00003496
UNSPSC代码:
12161700
eCl@ss:
38070211
PubChem化学物质编号:
57653505
NACRES:
NA.25

产品线

BioUltra

质量水平

100

表单

liquid

浓度

0.5 M in H2O

杂质

insoluble matter, passes filter test
≤0.001% total nitrogen (N)

pH值（酸碱度）

8.8-9.4 (25 °C, 0.5 M in H2O)

溶解性

H2O: 0.5 M at 20 °C, clear, colorless

密度

1.064 g/mL at 20 °C

痕量阴离子

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

痕量阳离子

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

0.5 M in H2O

紫外吸收

λ: 260 nm Amax: 0.019
λ: 280 nm Amax: 0.016

应用

diagnostic assay manufacturing

SMILES字符串

[Na+].[Na+].OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

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应用

磷酸氢二钠溶液可用于制备多种磷酸盐基生物缓冲液，用于细胞和分子生物学应用。
磷酸氢二钠溶液已用于：
  • 在钙化培养基中添加Dulbecco′改良伊格尔培养基（DMEM），以诱导血管平滑肌细胞（VSMC）的钙化
  • 制备CaCl2和 MgCl2的原液，以合成磷酸钙（CPB）和磷酸镁（MPB）
  • 补充2xYT琼脂板以培养BL21 DE3星型细胞和BL21dLac Gold型细胞

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储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

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分析证书(COA)

Lot/Batch Number
BCCM4374
BCCL9937
BCCM2829
BCCK4896
BCCJ7861

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Human molecular genetics, 24(9), 2470-2481 (2015-01-22)
Loss of gamma-sarcoglycan (γ-SG) induces muscle degeneration and signaling defects in response to mechanical load, and its absence is common to both Duchenne and limb girdle muscular dystrophies. Growing evidence suggests that aberrant signaling contributes to the disease pathology; however
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Journal of proteome research, 14(1), 120-132 (2014-10-21)
Interactions between a host and a bacterial pathogen are mediated by cross-talk between molecules present on, or secreted by, pathogens and host binding-molecules. Identifying proteins involved at this interface would provide substantial insights into this interaction. Although numerous studies have
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The rate of obesity among men of reproductive age has tripled in the last three decades. Previously, we demonstrated that paternal obesity resulted in impaired preimplantation developmental kinetics, compromised post-compaction metabolism and decreased blastocyst cell number when embryos were generated
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Disease models & mechanisms, 7(2), 299-309 (2014-04-10)
The skeleton is a preferred homing site for breast cancer metastasis. To date, treatment options for patients with bone metastases are mostly palliative and the disease is still incurable. Indeed, key mechanisms involved in breast cancer osteotropism are still only
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实验方案

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

醇脱氢酶（EC 1.1.1.1）的酶学测定

在测定醇脱氢酶活性时，该测定使用β-烟酰胺腺嘌呤二核苷酸磷酸和在340nm处的连续分光光度法进行测定。

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

透明质酸酶的酶法测定 (3.2.1.35)

在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

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