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质量水平
无菌性
sterile; sterile-filtered
形式
liquid
技术
cell culture | mammalian: suitable (suspension)
组分
NaHCO3: yes
HEPES: no
L-glutamine: no
glucose: yes
运输
ambient
储存温度
2-8°C
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应用
EX-CELL 293 是一种无动物蛋白、无血清培养基， 开发用于人胚肾细胞293（HEK 293）和相关细胞的长期生长。这些细胞， 为悬浮培养物，可以从补充血清的培养基转入EX-CELL 293直接进行继代培养， 无需改造或需要很少的改造。悬浮培养物可以 维持生长10天左右，无需再次接种， 并且可以传代20次以上而不损失存活率。
EX-CELL 293是一种不含动物蛋白的无血清培养基，用于人胚肾293（HEK 293）及相关细胞的长期生长。悬浮培养的细胞可以从补充血清培养基中直接传代培养到EX-CELL 293中，无需或仅需极少的适应期。悬浮培养物可以维持培养10天而无需换液，可传代20次以上而不损失存活率。
法律信息
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
动植物来源培养基
G Schoofs et al.
Cytotechnology, 28(1-3), 81-89 (2008-11-13)
We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum
Pratik Jaluria et al.
Biotechnology and bioengineering, 99(6), 1443-1452 (2007-11-21)
Animal-derived serum is an essential media supplement for mammalian cells in cell culture. For a number of reasons including cost, regulatory concerns, lot inconsistency, potential contamination with adventitious agents, and down-stream processing it is desirable to eliminate the use of
Jan Bláha et al.
Protein expression and purification, 109, 7-13 (2015-01-28)
Lectin-like transcript 1 (LLT1, gene clec2d) was identified to be a ligand for the single human NKR-P1 receptor present on NK and NK-T lymphocytes. Naturally, LLT1 is expressed on the surface of NK cells, stimulating IFN-γ production, and is up-regulated
Jan Bláha et al.
Protein expression and purification, 140, 36-43 (2017-08-02)
Human natural killer receptor protein 1 (NKR-P1, CD161, gene klrb1) is a C-type lectin-like receptor of natural killer (NK) cells responsible for recognition of its cognate protein ligand lectin-like transcript 1 (LLT1). NKR-P1 is the single human orthologue of the
J B Kaufman et al.
Cytotechnology, 33(1-3), 3-11 (2008-11-13)
The extracellular domain of human parathyroid Ca(2+) receptor was needed in order to study itsstructure and clinical application. The Ca(2+)receptor is a unique member of the G protein-coupledreceptor super-family, expressed in parathyroid andkidney cells where it has been shown to
商品
Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines.
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