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Merck
CN
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文件

05066

Sigma-Aldrich

琼脂糖

High EEO, for molecular biology

别名:

3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE

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About This Item

CAS号:
9012-36-6
EC 号:
232-731-8
MDL编号:
MFCD00081294
UNSPSC代码:
41105317
PubChem化学物质编号:
24845663
NACRES:
NA.25

等级

for molecular biology

质量水平

100

形式

powder

杂质

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

灼烧残渣

≤1%

缺失

≤10% loss on drying

EEO

0.23-0.27

转变温度

gel point 34-37 °C (1.5% solution)

凝胶强度

≥1500 g/cm2

痕量阴离子

chloride (Cl-): ≤0.3%
sulfate (SO42-): ≤0.6%

痕量阳离子

Ca: ≤0.05%
Cd: ≤0.001%
Co: ≤0.001%
Cr: ≤0.001%
Cu: ≤0.001%
Fe: ≤0.001%
K: ≤0.05%
Mg: ≤0.001%
Mn: ≤0.001%
Na: ≤0.5%
Ni: ≤0.001%
Pb: ≤0.001%
Zn: ≤0.001%

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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相关类别

应用

琼脂糖用于核酸的制备和分析分离。它可用于单细胞凝胶电泳测定。它还可用于southern印迹杂交。它也可用于评估来自培养的新生大鼠半圆形管上皮的跨上皮离子通量。

生化/生理作用

琼脂糖是一种多糖，用于500-20,000 bp的消退DNA和RNA片段。它提供了强大的凝胶结构，有助于更好的处理和更少的破裂。它还可以在琼脂糖凝胶培养过程中从去分化的软骨细胞重新表达分化的胶原表型。

包装

包装
50、500 g 聚乙烯瓶封装

分析说明

以下是与我们的琼脂糖相关的性质列表：
硫酸盐含量 - 用作纯度指标，因为硫酸盐是其中存在的主要离子基团。
凝胶强度 - 破裂凝胶所需施加的力。
胶凝点 - 琼脂糖水溶液在冷却时的胶凝温度。 琼脂糖溶液在液体-凝胶转变的过程中会表现出滞后现象 - 也就是说，它们的凝胶点与它们的熔点温度不同。
电内渗（EEO）- 液体通过凝胶的一种运动。琼脂糖凝胶中的阴离子基团被固定在基质上不能移动，但可解离的抗衡阳离子可以向基质中的阴极迁移，从而产生EEO。 由于生物聚合物的电泳运动通常是朝向阳极的，因此EEO会因为内部的对流而破坏分离。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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Molecular human reproduction, 2(8), 613-619 (1996-08-01)
Baseline DNA damage in spermatozoa from fertile and infertile men was compared using a modified alkali single cell gel electrophoresis (comet) assay. Semen from normozoospermic fertile, normozoospermic infertile and asthenozoospermic infertile (World Health Organization criteria, 1992) samples were studied. No
K C Reed et al.
Nucleic acids research, 13(20), 7207-7221 (1985-10-25)
The unique properties of nylon membranes allow for dramatic improvement in the capillary transfer of DNA restriction fragments from agarose gels (Southern blotting). By using 0.4 M NaOH as the transfer solvent following a short pre-treatment of the gel in
Aurica Precupas et al.
Chemical research in toxicology, 33(8), 2054-2071 (2020-07-01)
Understanding nanomaterial (NM)-protein interactions is a key issue in defining the bioreactivity of NMs with great impact for nanosafety. In the present work, the complex phenomena occurring at the bio/nano interface were evaluated in a simple case study focusing on
Pierre G Milhaud et al.
American journal of physiology. Cell physiology, 283(6), C1752-C1760 (2002-10-22)
The ductal epithelium of the semicircular canal forms much of the boundary between the K+-rich luminal fluid and the Na+-rich abluminal fluid. We sought to determine whether the net ion flux producing the apical-to-basal short-circuit current (I(sc)) in primary cultures
P D Benya et al.
Cell, 30(1), 215-224 (1982-08-01)
The differentiated phenotype of rabbit articular chondrocytes consists primarily of type II collagen and cartilage-specific proteoglycan. During serial monolayer culture this phenotype is lost and replaced by a complex collagen phenotype consisting predominately of type I collagen and a low
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