形式
powder
质量水平
组成
Agar, 15 g/L
Bacteriological peptone, 20 g/L
Glucose, 20 g/L
Yeast extract, 10 g/L
应用
food and beverages
适用性
nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.
一般描述
YPD琼脂是一种完整的酵母菌培养基，适合大多数酵母菌种，特别是酵母菌。它又称YEPD，包括酵母提取物、细菌性蛋白胨、葡萄糖和琼脂。这种培养基的成分为酵母的生长提供了所有必要的营养。培养基中必需的维生素、矿物质和氨基酸是碳氮源。
应用
YPD琼脂已作为培养基用于：
- 培育酿酒酵母菌株，为深空生物传感器任务找到长期保存的方法
- 基于最小抑菌浓度(MIC)的体外微量肉汤稀释(BMD)对酵母菌株的影响
- 用于Zymoseptori triciti菌株毒性研究
制备说明
1.取65 g溶于1L蒸馏水中。
2.在搅拌的同时加热至沸腾以完全溶解所有成分。
3.在121°C下高压灭菌15分钟。
2.在搅拌的同时加热至沸腾以完全溶解所有成分。
3.在121°C下高压灭菌15分钟。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
Ingrid A Edwards et al.
ACS infectious diseases, 3(12), 917-926 (2017-09-30)
Tachyplesin-1 (TP1; 1) is a cationic β-hairpin antimicrobial peptide with a membranolytic mechanism of action. While it possesses broad-spectrum, potent antimicrobial activity, 1 is highly hemolytic against mammalian erythrocytes, which precludes it from further development. In this study, we report
D A Treco et al.
Current protocols in molecular biology, Chapter 13, Unit13-Unit13 (2008-02-12)
Preparation of sterile media of consistently high quality is essential for the genetic manipulation of yeast. Recipes for media needed in the protocols in this chapter are provided in this unit. Specific suppliers are recommended for specific ingredients.
Xiao Liu et al.
Materials (Basel, Switzerland), 13(16) (2020-08-14)
Ammonia nitrogen and natural organic matter (NOM) seriously degrade the quality of surface waters. In this study, the optimum preparation conditions of a yeast-chitosan bio-microcapsule of the Candida tropicalis strain, used to treat micro-polluted surface water, were investigated. Fourier transform
Ilias Kounatidis et al.
G3 (Bethesda, Md.), 8(5), 1637-1647 (2018-03-15)
Candida glabrata (C. glabrata) forms part of the normal human gut microbiota but can cause life-threatening invasive infections in immune-compromised individuals. C. glabrata displays high resistance to common azole antifungals, which necessitates new treatments. In this investigation, we identified five
William T Kay et al.
Fungal genetics and biology : FG & B, 128, 14-19 (2019-03-17)
Zymoseptoria tritici, the causal agent of Septoria tritici blotch, is a notable pathogen of temperate-grown wheat. To better understand the mechanisms underpinning pathogenicity, leaf infection assays are commonly used to compare either the virulence of Z. tritici wildtype or mutant
实验方案
酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。酵母培养物可采用类似于细菌培养物的技术在液体培养基或琼脂平板上进行生长、维持和保存。
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
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