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Merck
CN

X114

Sigma-Aldrich

Triton X-114

laboratory grade

别名:

(1,1,3,3-四甲基丁基)苯基-聚乙二醇, 聚乙二醇叔辛基苯基醚

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About This Item

线性分子式:
(C2H4O)n C14H22O, n = 7 or 8
CAS号:
Beilstein:
2315025
MDL编号:
UNSPSC代码:
12161900
PubChem化学物质编号:
NACRES:
NA.21

等级

laboratory grade

质量水平

描述

non-ionic

分子量

average mol wt 537

组成

t-Oct-C6H4-(OCH2CH2)xOH, x = 7-8

折射率

n20/D 1.488 (lit.)

CMC

0.2 mM (20-25°C)

转变温度

pour point ~−9 °C
cloud point 23 °C

密度

1.058 g/mL at 25 °C (lit.)

HLB

12.4

SMILES字符串

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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一般描述

Triton X-114是一种非离子洗涤剂。该洗涤剂能够溶解膜,具有反相分离特性,可用于固有膜蛋白分离。Triton X-114溶液在0℃为均质状态,但20℃以上条件会发生相分离形成水相和洗涤剂相。蛋白质在Triton X-114溶液中的相分离已经过研究。辛基苯酚乙氧基化物和阴离子、阳离子和其他非离子表面活性剂兼容,在大多数酸性 &碱性溶液中化学性质稳定。

应用

Triton X-114已用于:
  • 通过相分离技术,从蛋白质溶液中去除内毒素
  • 从内毒素中提取和纯化DNA
  • 从细菌细胞壁二相提取脂蛋白
  • 一种低浊点(23 °C)的非离子除垢剂,能够溶解蛋白质,从两性蛋白相分离亲水蛋白

法律信息

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Y Aida et al.
Journal of immunological methods, 132(2), 191-195 (1990-09-14)
Endotoxin contamination of protein solutions was reduced by a phase separation technique using the detergent, Triton X-114. Protein solutions containing endotoxin were treated with Triton X-114 on ice. The solution was then warmed to 37 degrees C, whereupon two phases
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