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产品线
pHast Pack™
形式
powder
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一般描述
Phosphate Buffered Saline with 0.05% Tween® 20 or PBST contains PBS buffer with a low-concentration of Tween® detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.
应用
PBST is used in research in a variety of assays including DNA or RNA hybridization assays and cell culture. It is commonly used for immunoassays like Western blot, ELISA, Dot blot, Immunohistochemistry (IHC), immunopercipitation (IP), and immunofluorecence. It has been used for incubation and wash buffer for peptide microarrays for performing High-density peptide array (HDPA) to analyze antibody responses against SARS-CoV-2.
特点和优势
- Ready-to-use buffer
- No measuring and pH adjusting needed
- Save time and minimize effort
- Biological tests: free of DNase, RNase, protease, and nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
包装
Foil pouches
重悬
Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na2HPO4, 1.8 mM KH2PO4, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.
储存及稳定性
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
其他说明
For additional information on our range of Biochemicals, please complete this form.
法律信息
TWEEN is a registered trademark of Croda International PLC
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Selection for immune evasion in SARS-CoV-2 revealed by high-resolution epitope mapping and sequence analysis
iScience, 26, 107394-107394 (2023)
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