PPB005
Phosphate Buffered Saline
powder, pH 7.4, with 0.05% Tween® 20, pHast Pack™
别名:
PBS + 0.05% TWEEN® 20, PBS with 0.05% Tween 20, PBS-T, PBST
产品名称
磷酸盐缓冲剂，含0.05% TWEEN® 20，pH 7.4, pHast Pack™, powder
product line
pHast Pack™
form
powder
storage condition
(Tightly closed. Dry)
impurities
≤5 ppm Heavy Metals (lead)
color
white
pH
7.2—7.6 (25 °C)
solubility
soluble, clear to hazy, colorless
cation traces
Fe: <10 ppm
foreign activity
DNase, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNase, none detected
storage temp.
room temp
Quality Level
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Application
PBST is used in research in a variety of assays including DNA or RNA hybridization assays and cell culture. It is commonly used for immunoassays like Western blot, ELISA, Dot blot, Immunohistochemistry (IHC), immunopercipitation (IP), and immunofluorecence. It has been used for incubation and wash buffer for peptide microarrays for performing High-density peptide array (HDPA) to analyze antibody responses against SARS-CoV-2.
Features and Benefits
- Ready-to-use buffer
- No measuring and pH adjusting needed
- Save time and minimize effort
- Biological tests: free of DNase, RNase, protease, and nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
General description
Phosphate-buffered saline with 0.05% Tween® 20 or PBST contains PBS buffer with a low-concentration of Tween detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Packaging
Foil pouches
Preparation Note
Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na2HPO4, 1.8 mM KH2PO4, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Legal Information
TWEEN is a registered trademark of Croda International PLC
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Selection for immune evasion in SARS-CoV-2 revealed by high-resolution epitope mapping and sequence analysis
N'Guessan et al.
iScience, 26, 107394-107394 (2023)
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