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Merck
CN

51976

Sigma-Aldrich

Ethyl alcohol, pure

≥99.8%, for molecular biology, (absolute alcohol, without additive, A15 o1), BioUltra

别名:

乙醇

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About This Item

线性分子式:
CH3CH2OH
CAS号:
分子量:
46.07
Beilstein:
1718733
EC 号:
MDL编号:
UNSPSC代码:
12191601
PubChem化学物质编号:
NACRES:
NA.25

product name

酒精, BioUltra, for molecular biology, ≥99.8%, (absolute alcohol, without additive, A15 o1)

等级

for molecular biology

质量水平

蒸汽密度

1.59 (vs air)

蒸汽压

44.6 mmHg ( 20 °C)

产品线

BioUltra

检测方案

≥99.8%

形式

liquid

自燃温度

683 °F

质量

(absolute alcohol, without additive, A15 o1)

expl. lim.

19 %, 60 °F

杂质

DNases, none detected
KMnO4-reducing substances, in accordance
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
substances darkened by H2SO4, in accordance
≤0.0004% free alkali (as NH3)
≤0.001% aldehyde (as CH3CHO)
≤0.001% formaldehyde (as HCHO)
≤0.001% ketone (as CH3COCH3)
≤0.002% free acid (as CH3COOH)
≤0.1% methanol (CH3OH)
≤0.2% water

蒸发残留物

≤0.001%

折射率

n20/D 1.3600 (lit.)
n20/D 1.362

bp

78 °C (lit.)

mp

−114 °C (lit.)

溶解性

H2O: miscible
organic solvents: miscible

密度

0.789 g/mL at 25 °C (lit.)

痕量阳离子

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.1 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤1 mg/kg
Ni: ≤0.02 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

SMILES字符串

CCO

λ

neat

紫外吸收

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.03

适用性

suitable for molecular biology

应用

life science and biopharma

格式

neat

InChI

1S/C2H6O/c1-2-3/h3H,2H2,1H3

InChI key

LFQSCWFLJHTTHZ-UHFFFAOYSA-N

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一般描述

乙醇也称乙基醇,是一种直链结构的挥发性伯醇。常用作生化试剂,可使细菌、真菌和病毒来源的蛋白质有效变性。该乙醇不含DNA酶、RNA酶、切口酶和蛋白酶,在核酸(DNA & RNA)和蛋白等生物分子纯化和沉淀中发挥重要作用,广泛适用于细胞生物学、分子生物学和生物化学研究。此外,也适用在组织学中配制染色和脱色试剂,并用于组织包埋前的脱水。

应用

乙醇已被用于:
  • 进行小鼠实验所需的溶媒对照
  • 在刺激后和结晶紫一起对人和小鼠高级别浆液性卵巢癌(HGSOC)细胞染色进行活力检测
  • 用于制备细胞培养实验中使用的17β-雌二醇(E2)
  • 在细胞周期分析中用于固定细胞

生化/生理作用

乙醇是在工业和消费品中广泛使用的有机化学品。这种脂肪醇主要用作工业中的溶剂和其它化学品生产中的中间体。

特点和优势

  • 用途广泛,适应各种实验室和研究应用
  • BioUltra级试剂,适合分子生物学、细胞生物学和生物化学研究
  • 经检测确认,重金属污染水平低,适用性广
  • 不含DNA酶、RNA酶、切口酶和蛋白酶检测了是否存在微量水平的阴离子和阳离子

其他说明

如需了解生化试剂系列的更多信息,请填写此表
可能适用相应的销售限制。适用于核酸的沉淀。

附注

1在瑞士分发和使用乙醇需要瑞士联邦酒精管理局的许可证。

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象形图

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警示用语:

Danger

危险声明

危险分类

Eye Irrit. 2 - Flam. Liq. 2

WGK

WGK 1

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

危险化学品

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分析证书(COA)

Lot/Batch Number

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G M Huang et al.
Analytical biochemistry, 223(1), 35-38 (1994-11-15)
Genome projects require a high-throughput method for DNA template preparation, which traditional protocols can rarely provide. We introduce here a new protocol for plasmid and cosmid DNA preparation based on alkaline denaturation in a 96-well format. The essential improvement made
M G Mahoney et al.
The Journal of clinical investigation, 103(4), 461-468 (1999-02-18)
Patients with pemphigus foliaceus (PF) have blisters on skin, but not mucous membranes, whereas patients with pemphigus vulgaris (PV) develop blisters on mucous membranes and/or skin. PF and PV blisters are due to loss of keratinocyte cell-cell adhesion in the
Y Gavrieli et al.
The Journal of cell biology, 119(3), 493-501 (1992-11-01)
Programmed cell death (PCD) plays a key role in developmental biology and in maintenance of the steady state in continuously renewing tissues. Currently, its existence is inferred mainly from gel electrophoresis of a pooled DNA extract as PCD was shown
D M Lovinger et al.
Science (New York, N.Y.), 243(4899), 1721-1724 (1989-03-31)
The ion current induced by the glutamate receptor agonist N-methyl-D-aspartate (NMDA) in voltage-clamped hippocampal neurons was inhibited by ethanol (EtOH). Inhibition increased in a concentration-dependent manner over the range 5 to 50 mM, a range that also produces intoxication. The
Preparation and properties of serum and plasma proteins; crystallization of serum albumins from ethanol water mixtures.
E J COHN et al.
Journal of the American Chemical Society, 69(7), 1753-1761 (1947-07-01)

商品

Substances are said to be miscible in one another if they dissolve to form a uniform solution. Bookmark or download our miscibility table for common lab solvents.

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