ligand
iminodiacetic acid
质量水平
描述
for metal affinity chromatography (IMAC), suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
无菌性
sterile (Caustic Stable)
形式
resin
纯化方式
immobilized metal affinity chromatography (IMAC)
制造商/商品名称
Fractogel®
参数
8 bar max. pressure
~240 cm/hr flow rate
基质活性基团
methacrylate
wide
40-90 μm
容量
~60 mg binding capacity( lysozyme/ml of resin)
转变温度
flash point 35 °C (calculated)
应用
recombinant protein
分离技术
affinity
储存温度
2-30°C
一般描述
Fractogel® EMD Chelate combines our tentacle structure with iminodiacetic acid as the metal chelating ligand
特点和优势
Fractogel® EMD Chelate (M) enables users to:
- Efficiently purify histidine-tagged recombinant proteins
- Coordinate metal ions to non-bonding electron pairs of the amino acid side chains and separate peptides
- Ensure timely and successful startup and operation
- Minimize hydrophobic interactions due to the tentacles technology, resulting in superior selectivity and recovery
- Handle multiple CIP and packing cycles with the chemically and physically robust bead, leading to a longer resin lifetime
包装
- 1.10338.0100: Fractogel® EMD Chelate (M) Resin 100ml
- 1.10338.0010: Fractogel® EMD Chelate (M) Resin 10 ml
- 1.10338.0500: Fractogel® EMD Chelate (M) Resin 500 ml
- 1.10338.5000: Fractogel® EMD Chelate (M) Resin 5 L
Plastic bottle
分析说明
Appearance: Milky tubid suspension, free from impurities (foreigen particles)
Microscopic evaluation: Uniform spherical particles, no agglomerates, no fines
Cerium: ≤ 10 µg/g
Copper-(II)-binding capacity: 60 - 100 µmol/ml
Extractable matter (water): ≤ 0.05 %
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Functional test: passes test
Functional test: Separation of chymotrypsinogen A and myoglobin
Microscopic evaluation: Uniform spherical particles, no agglomerates, no fines
Cerium: ≤ 10 µg/g
Copper-(II)-binding capacity: 60 - 100 µmol/ml
Extractable matter (water): ≤ 0.05 %
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Functional test: passes test
Functional test: Separation of chymotrypsinogen A and myoglobin
法律信息
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Warning
危险声明
危险分类
Flam. Liq. 3
WGK
WGK 1
闪点(°F)
95.0 °F
闪点(°C)
35 °C
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门