Home Sample PurificationConverting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

It is convenient when comparing results for columns of different sizes to express ﬂow as linear ﬂow (cm/hour). However, ﬂow is usually measured in volumetric ﬂow rate (mL/min).

To convert between linear ﬂow and volumetric ﬂow rate use one of the formulae below.

From linear ﬂow (cm/hour) to volumetric ﬂow rate (mL/min)

Volumetric ﬂow rate (ml/min) =

Linear ﬂow (cm/h)

x column cross sectional area (cm²)

π x d²

where
Y = linear flow in cm/h
d = column inner diameter in cm

Example:
What is the volumetric flow rate in an XK 16/70 column (i.d. 1.6 cm) when the linear flow is 150 cm/hour?
Y = linear flow = 150 cm/h
d = inner diameter of the column = 1.6 cm

Volumetric ﬂow rate (ml/min) =

150 × π × 1.6 x 1.6

60 × 4

mL/min

= 5.03 ml/min

From volumetric flow rate (mL/min) to linear flow (cm/hour)

Linear ﬂow rate =

Volumetric ﬂow rate (ml/min) × 60

column cross sectional area (cm²)

= Z × 60 ×

π x d²

Z = volumetric flow rate in mL/min
d = column inner diameter in cm

Example:
What is the linear flow in an HR 5/5 column (i.d. 0.5 cm) when the volumetric flow rate is 1 mL/min?
Z = Volumetric flow rate = 1 mL/min
d = column inner diameter = 0.5 cm

Linear ﬂow = 1 × 60 ×

π x 0.5 x 0.5

cm/h

= 305.6 cm/h

From mL/min to using a syringe

1 mL/min = approximately 30 drops/min on a HiTrap 1 mL column
5 mL/min = approximately 120 drops/min on a HiTrap 5 mL column

Materials

Product Number	Product Name	Product Description
703168	顺式-双(异硫氰基)(2,2′-联吡啶基-4,4′-二羧基)(4,4′-二-壬基-2′-联吡啶基)钌(II)	95% (NMR)
703206	顺 -双（异硫氰酸盐）双（2,2′-联吡啶-4,4′-二羧酸钌 (II)
703214	二-四丁铵顺式-双(异硫氰基)双(2,2′-联吡啶-4,4′-二羧基)钌(II)	95% (NMR)
636916	氧化铟锡涂覆的矩形玻片	surface resistivity 15-25 Ω/sq, slide
636908	氧化铟锡涂覆的矩形玻片	surface resistivity 30-60 Ω/sq, slide
65290	3-甲氧基丙腈	≥98.0% (GC)
718467	氧化钛（IV）	nanopowder, 21 nm primary particle size (TEM), ≥99.5% trace metals basis
637254	氧化钛 (IV)，锐钛矿	nanopowder, <25 nm particle size, 99.7% trace metals basis
634662	氧化钛(IV)，金红石和锐钛矿混合物	nanopowder, <100 nm particle size (BET), 99.5% trace metals basis
700347	氧化钛(IV)，金红石和锐钛矿混合物	nanoparticles, <150 nm particle size (volume distribution, DLS), dispersion, 40 wt. % in H₂O, 99.5% trace metals basis
700339	氧化钛(IV)，金红石和锐钛矿混合物	nanoparticles, <100 nm particle size, dispersion, 48-52 wt. % in xylene, 99.9% trace metals basis
700355	氧化钛(IV)，金红石和锐钛矿混合物	nanoparticle, <250 nm particle size (DLS), paste, 53-57 wt. % in diethylene glycol monobutyl ether/ethylene glycol, 99.9% trace metals basis
637262	钛 (IV) 氧化物，金红石型	nanopowder, <100 nm particle size, 99.5% trace metals basis

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