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HPLC Analysis of Vitamins, Fat Soluble (D2 and D3), on Discovery® HS C18

HPLC Analysis of Vitamins, Fat Soluble (D2 and D3), on Discovery® HS C18 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

569252-U

Discovery® HS C18 HPLC Column

3 μm particle size, L × I.D. 15 cm × 4.6 mm

standard

Product No.
Description
Pricing

47763

Cholecalciferol (D3)

analytical standard

47768

Ergocalciferol (D2)

analytical standard

CONDITIONS

column

Discovery HS C18, 15 cm × 4.6 mm I.D., 3 μm particles (569252-U)

mobile phase

100% acetonitrile

flow rate

0.8 mL/min

column temp.

30 °C

detector

UV, 290 nm

injection

10 μL

sample

50 μg/mL each analyte

Description

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Related Product Categories

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Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS