HPLC Analysis of cis/trans-Dothiepin Enantiomers on Astec® CYCLOBOND™ I 2000 AC
Materials
analytical column
Product No.
Description
Pricing
CONDITIONS
column
CYCLOBOND I 2000 AC, 25 cm x 4.6 mm I.D., 5 μm particles (20124AST)
mobile phase
[A] 1.0% triethylamine acetate, pH 4.1; [B] acetonitrile; (85:15, A:B)
flow rate
1 mL/min
column temp.
22 °C
detector
UV, 254 nm
injection
2 μL
sample
1 mg/mL in methanol
peak 1: 9.48 min.
peak 2: 12.48 min.
Description
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CYCLOBOND is a trademark of Sigma-Aldrich Co. LLC