HPLC Analysis of Atrazine Herbicides on Ascentis® Express C18
Materials
analytical column
Product No.
Description
Pricing
standard
Product No.
Description
Pricing
CONDITIONS
column
Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)
mobile phase
[A] 20 mM ammonium acetate, pH 6.4 unadjusted; [B] acetonitrile
gradient
20 to 28% B in 11 min; 28 to 65% B in 5 min; held at 65% B for 4 min
flow rate
0.6 mL/min
column temp.
46 °C
detector
UV, 240 nm
injection
5 μL
sample
10-25 μg/mL in 10:90, water: acetonitrile
Description
Analysis Note
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of 17 triazine pesticides.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany