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  • Comparison of the cellular transport mechanism of cationic, star-shaped polymers and liposomes in HaCat cells.

Comparison of the cellular transport mechanism of cationic, star-shaped polymers and liposomes in HaCat cells.

International journal of nanomedicine (2017-02-23)
Heng-Cong Luo, Na Li, Li Yan, Kai-Jin Mai, Kan Sun, Wei Wang, Guo-Juan Lao, Chuan Yang, Li-Ming Zhang, Meng Ren
ABSTRACT

Several biological barriers must be overcome to achieve efficient nonviral gene delivery. These barriers include target cell uptake, lysosomal degradation, and dissociation from the carrier. In this study, we compared the differences in the uptake mechanism of cationic, star-shaped polymer/MMP-9siRNA complexes (β-CD-(D3)7/MMP-9siRNA complexes: polyplexes) and commercial liposome/MMP-9siRNA complexes (Lipofectamine

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methyl-β-cyclodextrin, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
MISSION® esiRNA, targeting human MMP9