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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium chloride, tested according to Ph. Eur.
Sigma-Aldrich
Sodium chloride solution, BioUltra, for molecular biology, ~5 M in H2O
Supelco
1-Octanol, analytical standard
Supelco
Tetrahydrofuran, Selectophore, ≥99.5%
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Methanol, analytical standard
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N-Ethyldiisopropylamine solution, suitable for peptide synthesis, ~2 M in 1-methyl-2-pyrrolidinone
Sigma-Aldrich
Sodium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
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N,N-Dimethylformamide, analytical standard
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Acetone, analytical standard
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Diethylene glycol, ReagentPlus®, 99%
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Piperidine, ReagentPlus®, 99%
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Methanol, anhydrous, 99.8%
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N,N-Dimethylformamide, anhydrous, 99.8%
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Triphenyl phosphite, 97%
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Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free
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Acetone, natural, ≥97%
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Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
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Sodium chloride-35Cl, 99 atom % 35Cl
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Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
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Acetone, ≥99%, meets FCC analytical specifications
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1-Octanol, natural, ≥98%, FCC
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Formaldehyde solution, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
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1-Octanol, ≥98%, FCC, FG
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Formaldehyde solution, for molecular biology, BioReagent, ≥36.0% in H2O (T)
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Diethylene glycol, puriss. p.a., ≥99.0% (GC), colorless
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Tetraethyl orthosilicate, ≥99.0% (GC)
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Diethylene glycol, BioUltra, ≥99.0% (GC)
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1-Octanol, anhydrous, ≥99%
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Tetrahydrofuran, anhydrous, contains 250 ppm BHT as inhibitor, ≥99.9%
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Sodium chloride solution, 0.85%