DNA duplex stabilization in crowded polyanion solutions.

DNA duplex stabilization in crowded polyanion solutions.

Chemical communications (Cambridge, England) (2013-01-11)

Imran Khimji, Jeehae Shin, Juewen Liu

ABSTRACT

The melting temperature of duplex DNA is much higher in polyanions than in non-ionic polymers with similar ionic strength, suggesting an additional electrostatic contribution on top of the excluded volume effect.

MATERIALS

Product Number

Brand

Product Description

523925

Sigma-Aldrich

Poly(acrylic acid) solution, average M_w ~100,000, 35 wt. % in H₂O

416010

Sigma-Aldrich

Poly(acrylic acid, sodium salt) solution, average M_w ~1,200, 45 wt. % in H₂O

535931

Sigma-Aldrich

Poly(acrylic acid) solution, average M_w ~2,000, 50 wt. % in H₂O, electronic grade

416029

Sigma-Aldrich

Poly(acrylic acid, sodium salt) solution, average M_w ~8,000, 45 wt. % in H₂O

416002

Sigma-Aldrich

Poly(acrylic acid) solution, average M_w ~250,000, 35 wt. % in H₂O

81123

Supelco

Poly(acrylic acid sodium salt), analytical standard, for GPC, 16,000

447013

Sigma-Aldrich

Poly(acrylic acid sodium salt), average M_w ~5,100 by GPC, powder

323667

Sigma-Aldrich

Poly(acrylic acid)

306215

Sigma-Aldrich

Poly(acrylic acid), average M_v ~1,250,000

306231

Sigma-Aldrich

Poly(acrylic acid), average M_v ~4,000,000

306223

Sigma-Aldrich

Poly(acrylic acid), average M_v ~3,000,000

181293

Supelco

Poly(acrylic acid), analytical standard, average M_n 130,000 (Typical)

420344

Sigma-Aldrich

Poly(acrylic acid sodium salt), average M_w ~2,100

181285

Sigma-Aldrich

Poly(acrylic acid), average M_v ~450,000