- Reduced NGF secretion by HT-29 human colon cancer cells treated with a GRPR antagonist.
Reduced NGF secretion by HT-29 human colon cancer cells treated with a GRPR antagonist.
Protein and peptide letters (2009-06-13)
Caroline Brunetto de Farias, Laura Stertz, Rodrigo Cruz Lima, Flávio Kapczinski, Gilberto Schwartsmann, Rafael Roesler
PMID19519524
ABSTRACT
The gastrin-releasing peptide receptor (GRPR) is a therapeutic target in colon cancer. Here we show that the GRPR antagonist RC-3095 (10(-3), 10(-6), or 1 microM) decreases nerve growth factor (NGF) secretion measured by enzyme-linked immunosorbent assay (ELISA) in HT-29 human colon carcinoma cells. The results suggest that decreased secretion of neurotrophins might be a novel mechanism by which GRPR antagonists exert their antiproliferative effects in cancer cells.
MATERIALS