- Protein Expression Protocol for the Catalytic Domain of an Adenylate Cyclase Membrane-Anchored by a Vibrio Quorum Sensing Receptor.
Protein Expression Protocol for the Catalytic Domain of an Adenylate Cyclase Membrane-Anchored by a Vibrio Quorum Sensing Receptor.
Bio-protocol (2017-01-20)
Stephanie Beltz, Joachim E Schultz
PMID34458439
ABSTRACT
The direct regulation of a mycobacterial adenylate cyclase (Rv1625c) via exchange of its membrane anchor by the quorum sensing receptor CqsS (Vibrio harveyi) has recently been reported ( Beltz et al., 2016 ). This protocol describes the expression and membrane preparation for these chimeric proteins.