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  • CX3CR1+ CD115+ CD135+ common macrophage/DC precursors and the role of CX3CR1 in their response to inflammation.

CX3CR1+ CD115+ CD135+ common macrophage/DC precursors and the role of CX3CR1 in their response to inflammation.

The Journal of experimental medicine (2009-03-11)
Cedric Auffray, Darin K Fogg, Emilie Narni-Mancinelli, Brigitte Senechal, Celine Trouillet, Noah Saederup, Julia Leemput, Karine Bigot, Laura Campisi, Marc Abitbol, Thierry Molina, Israel Charo, David A Hume, Ana Cumano, Gregoire Lauvau, Frederic Geissmann
ABSTRACT

CX(3)CR1 expression is associated with the commitment of CSF-1R(+) myeloid precursors to the macrophage/dendritic cell (DC) lineage. However, the relationship of the CSF-1R(+) CX(3)CR1(+) macrophage/DC precursor (MDP) with other DC precursors and the role of CX(3)CR1 in macrophage and DC development remain unclear. We show that MDPs give rise to conventional DCs (cDCs), plasmacytoid DCs (PDCs), and monocytes, including Gr1(+) inflammatory monocytes that differentiate into TipDCs during infection. CX(3)CR1 deficiency selectively impairs the recruitment of blood Gr1(+) monocytes in the spleen after transfer and during acute Listeria monocytogenes infection but does not affect the development of monocytes, cDCs, and PDCs.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Triton X-100, for molecular biology
Sigma-Aldrich
Triton X-100, peroxide- and carbonyl-free
Sigma-Aldrich
Triton X-100, laboratory grade
Sigma-Aldrich
Triton X-100, BioXtra