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V900483

Sigma-Aldrich

Trizma® base

≥99%, crystalline powder, reagent grade, Vetec™

Synonym(s):

2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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About This Item

Linear Formula:
NH2C(CH2OH)3
CAS Number:
77-86-1
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
201-064-4
MDL number:
MFCD00004679
UNSPSC Code:
12161700
PubChem Substance ID:
329829884

product name

Trizma® base, Vetec™, reagent grade, ≥99%

grade

reagent grade

description

aminopeptidase substrate

product line

Vetec™

Assay

≥99%

form

crystalline powder

pH

10.5-12

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: 0.667 g/mL, clear, colorless to faintly yellow

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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General description

Trizma® base is a basimetric standard used in biochemistry and molecular biology. It may be used as a buffer or as a component of mixed buffer formulations. It is essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Trizma® base can be used as a buffer:
  • In the evanescent absorption analysis of NADPH.
  • In the autoxidation studies of oxymyoglobin.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Vetec is a trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Autoxidation of native oxymyoglobin. Kinetic analysis of the pH profile
Shikama K and Sugawara Y
European Journal of Biochemistry, 91(2), 407-413 (1978)
Tapered fibers for cell studies
Reviews in Fluorescence (2005)
Preparation of buffers for use in enzyme studies
Handbook of Biochemistry and Molecular Biology (2010)
Tri (hydroxymethyl) aminomethane as Acidimetric Standard.
Fossum J, et al.
Analytical Chemistry, 23(3), 491-493 (1951)
Corey A Baron et al.
Magnetic resonance in medicine, 73(3), 1075-1084 (2014-04-12)
An acquisition method that does not increase scan time or specific absorption rate is investigated for reducing the deleterious effects of cerebrospinal fluid (CSF) partial volume effects on diffusion tensor imaging (DTI) tractography. It is based on using a shorter
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