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V900468

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

Vetec, reagent grade, ≥98%

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Synonym(s):
5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal
Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
Molecular Weight:
408.63
Beilstein:
1402009
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:

grade

reagent grade

product line

Vetec

Assay

≥98%

form

powder

solubility

DMF: methanol (1:1): 50 mg/mL, clear, colorless to light yellow

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

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Application

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Legal Information

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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