G1007615
LC/MS Analysis of Nucleotides on SeQuant® ZIC-cHILIC
application for LC-MS
technique(s)
LC/MS: suitable
test parameters
column: SeQuant® ZIC-cHILIC, 10 cm x 2.1 mm, 3.0 μm particles (1.50657)
mobile phase: [A] acetonitrile; [B] 50 mM ammonium acetate, pH 5.0 with acetic acid in water
gradient: 26 to 27% B in 10 min; to 27 to 35% B in 10 min; to 26% B in 0.1 min; hold at 26% B for 9.9 min
flow rate: 0.3 mL/min
pressure: 1029 psi (71 bar)
column temp.: 50 °C
detector: Q-TOF (ESI-negative), XIC of the following m/z values: AMP-346, ADP-426, ATP-506, CMP-322, CDP-402, (CTP-482, GMP-362, GDP-442, GTP-522, UMP-323, UDP-403, UTP-483)
injection: 10 μL
sample: 10-30 μg/mL of nucleotides (AMP, ADP, ATP, CMP, CDP, CTP, GMP, GDP, GTP, UMP, UDP, UTP) (80/20 acetonitrile/50 mM ammonium acetate in water, pH 5.3 with acetic acid)
suitability
application for LC-MS
application(s)
pharmaceutical (small molecule)
Analysis Note
Legal Information
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standard
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