G006124
HPLC Analysis of Salicylic Acid and Acetylsalicylic Acid (Aspirin) on Ascentis® Express OH5
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express OH5, 10 cm x 3.0 mm I.D., 2.7 μm particles (53769-U)
mobile phase: 2 mM ammonium acetate in 10:90 water:acetonitrile, pH to 7.0 with acetic acid, glacial
flow rate: 0.5 mL/min
pressure: 850 psi (59 bar)
column temp.: 35 °C
detector: UV, 235 nm
injection: 2 μL
sample: 100 μg/mL salicylic acid, 200 μg/mL acetylsalicylic acid in methanol:acetonitrile (3:7)
suitability
application for HPLC
application(s)
pharmaceutical (small molecule)
Legal Information
Analyte
analytical column
standard
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